LOCUS       sequence-503105-       15389 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  PopuA+160xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15389)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 15389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB161
FEATURES             Location/Qualifiers
     source          1..15389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2863..2881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2910..2928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2959..2977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2991..3009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3079..3097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3121..3139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3169..3187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3218..3236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3260..3278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3306..3324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3348..3366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3396..3414
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3445..3463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3487..3505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3550..3568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3597..3615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3646..3664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3678..3696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3720..3738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3766..3784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3808..3826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3856..3874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3905..3923
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3947..3965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4010..4028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4057..4075
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4106..4124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4138..4156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4180..4198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4226..4244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4268..4286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4316..4334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4365..4383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4407..4425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4470..4488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4517..4535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4566..4584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4598..4616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4640..4658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4686..4704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4728..4746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4776..4794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4825..4843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4867..4885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4930..4948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4977..4995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5026..5044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5058..5076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5100..5118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5146..5164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5188..5206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5236..5254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5285..5303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5327..5345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5390..5408
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5437..5455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5486..5504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5518..5536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5560..5578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5606..5624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5648..5666
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5696..5714
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5745..5763
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5787..5805
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5850..5868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5897..5915
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5946..5964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5978..5996
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6020..6038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6066..6084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6108..6126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6156..6174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6205..6223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6247..6265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6310..6328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6357..6375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6406..6424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6438..6456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6480..6498
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6526..6544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6568..6586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6616..6634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6665..6683
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6707..6725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6770..6788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6817..6835
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6866..6884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6898..6916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6940..6958
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6986..7004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7028..7046
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7076..7094
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7125..7143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7167..7185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7230..7248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7277..7295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7326..7344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7358..7376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7400..7418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7446..7464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7488..7506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7536..7554
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7585..7603
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7627..7645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7690..7708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7737..7755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7786..7804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7818..7836
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7860..7878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7906..7924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7948..7966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7996..8014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8045..8063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8087..8105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      8140..8211
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8227..8254
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             8792..9415
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(8865..8884)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    9580..9598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(9685..9687,9688..9690,9691..10404)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(9730..9751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      10425..10452
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(12432..13223,13224..13292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13055..13074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14584..14972
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            15143..15252
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     2881 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     2941 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     3001 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     3061 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     3121 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     3181 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     3241 tttcaccgct ggcgtcaatt ccctatcagt gatagagata ggctcctttg tcaaaagtga
     3301 tcgcttccct atcagtgata gagacttcac gggataatgt gttctactcc ctatcagtga
     3361 tagagagctt aagtcaatcc aatgcgttca ggtgctccct atcagtgata gagaaactcc
     3421 gtcatcgtgg atgtcaatac tgactcccta tcagtgatag agattgattt caccgctggc
     3481 gtcaattccc tatcagtgat agagacttag gtgtccctag aattactaga gatcgatttt
     3541 agagctgcct ccctatcagt gatagagatt gctacaactg caaggtagtc ccggtatccc
     3601 tatcagtgat agagactgat agctacgagt ctgagatcct acttgtccct atcagtgata
     3661 gagacagtac cgagttttcc ctatcagtga tagagagctc atactgcgac agagtctttt
     3721 ccctatcagt gatagagata ggctcctttg tcaaaagtga tcgcttccct atcagtgata
     3781 gagacttcac gggataatgt gttctactcc ctatcagtga tagagagctt aagtcaatcc
     3841 aatgcgttca ggtgctccct atcagtgata gagaaactcc gtcatcgtgg atgtcaatac
     3901 tgactcccta tcagtgatag agattgattt caccgctggc gtcaattccc tatcagtgat
     3961 agagacttag gtgtccctag aattactaga gatcgatttt agagctgcct ccctatcagt
     4021 gatagagatt gctacaactg caaggtagtc ccggtatccc tatcagtgat agagactgat
     4081 agctacgagt ctgagatcct acttgtccct atcagtgata gagacagtac cgagttttcc
     4141 ctatcagtga tagagagctc atactgcgac agagtctttt ccctatcagt gatagagata
     4201 ggctcctttg tcaaaagtga tcgcttccct atcagtgata gagacttcac gggataatgt
     4261 gttctactcc ctatcagtga tagagagctt aagtcaatcc aatgcgttca ggtgctccct
     4321 atcagtgata gagaaactcc gtcatcgtgg atgtcaatac tgactcccta tcagtgatag
     4381 agattgattt caccgctggc gtcaattccc tatcagtgat agagacttag gtgtccctag
     4441 aattactaga gatcgatttt agagctgcct ccctatcagt gatagagatt gctacaactg
     4501 caaggtagtc ccggtatccc tatcagtgat agagactgat agctacgagt ctgagatcct
     4561 acttgtccct atcagtgata gagacagtac cgagttttcc ctatcagtga tagagagctc
     4621 atactgcgac agagtctttt ccctatcagt gatagagata ggctcctttg tcaaaagtga
     4681 tcgcttccct atcagtgata gagacttcac gggataatgt gttctactcc ctatcagtga
     4741 tagagagctt aagtcaatcc aatgcgttca ggtgctccct atcagtgata gagaaactcc
     4801 gtcatcgtgg atgtcaatac tgactcccta tcagtgatag agattgattt caccgctggc
     4861 gtcaattccc tatcagtgat agagacttag gtgtccctag aattactaga gatcgatttt
     4921 agagctgcct ccctatcagt gatagagatt gctacaactg caaggtagtc ccggtatccc
     4981 tatcagtgat agagactgat agctacgagt ctgagatcct acttgtccct atcagtgata
     5041 gagacagtac cgagttttcc ctatcagtga tagagagctc atactgcgac agagtctttt
     5101 ccctatcagt gatagagata ggctcctttg tcaaaagtga tcgcttccct atcagtgata
     5161 gagacttcac gggataatgt gttctactcc ctatcagtga tagagagctt aagtcaatcc
     5221 aatgcgttca ggtgctccct atcagtgata gagaaactcc gtcatcgtgg atgtcaatac
     5281 tgactcccta tcagtgatag agattgattt caccgctggc gtcaattccc tatcagtgat
     5341 agagacttag gtgtccctag aattactaga gatcgatttt agagctgcct ccctatcagt
     5401 gatagagatt gctacaactg caaggtagtc ccggtatccc tatcagtgat agagactgat
     5461 agctacgagt ctgagatcct acttgtccct atcagtgata gagacagtac cgagttttcc
     5521 ctatcagtga tagagagctc atactgcgac agagtctttt ccctatcagt gatagagata
     5581 ggctcctttg tcaaaagtga tcgcttccct atcagtgata gagacttcac gggataatgt
     5641 gttctactcc ctatcagtga tagagagctt aagtcaatcc aatgcgttca ggtgctccct
     5701 atcagtgata gagaaactcc gtcatcgtgg atgtcaatac tgactcccta tcagtgatag
     5761 agattgattt caccgctggc gtcaattccc tatcagtgat agagacttag gtgtccctag
     5821 aattactaga gatcgatttt agagctgcct ccctatcagt gatagagatt gctacaactg
     5881 caaggtagtc ccggtatccc tatcagtgat agagactgat agctacgagt ctgagatcct
     5941 acttgtccct atcagtgata gagacagtac cgagttttcc ctatcagtga tagagagctc
     6001 atactgcgac agagtctttt ccctatcagt gatagagata ggctcctttg tcaaaagtga
     6061 tcgcttccct atcagtgata gagacttcac gggataatgt gttctactcc ctatcagtga
     6121 tagagagctt aagtcaatcc aatgcgttca ggtgctccct atcagtgata gagaaactcc
     6181 gtcatcgtgg atgtcaatac tgactcccta tcagtgatag agattgattt caccgctggc
     6241 gtcaattccc tatcagtgat agagacttag gtgtccctag aattactaga gatcgatttt
     6301 agagctgcct ccctatcagt gatagagatt gctacaactg caaggtagtc ccggtatccc
     6361 tatcagtgat agagactgat agctacgagt ctgagatcct acttgtccct atcagtgata
     6421 gagacagtac cgagttttcc ctatcagtga tagagagctc atactgcgac agagtctttt
     6481 ccctatcagt gatagagata ggctcctttg tcaaaagtga tcgcttccct atcagtgata
     6541 gagacttcac gggataatgt gttctactcc ctatcagtga tagagagctt aagtcaatcc
     6601 aatgcgttca ggtgctccct atcagtgata gagaaactcc gtcatcgtgg atgtcaatac
     6661 tgactcccta tcagtgatag agattgattt caccgctggc gtcaattccc tatcagtgat
     6721 agagacttag gtgtccctag aattactaga gatcgatttt agagctgcct ccctatcagt
     6781 gatagagatt gctacaactg caaggtagtc ccggtatccc tatcagtgat agagactgat
     6841 agctacgagt ctgagatcct acttgtccct atcagtgata gagacagtac cgagttttcc
     6901 ctatcagtga tagagagctc atactgcgac agagtctttt ccctatcagt gatagagata
     6961 ggctcctttg tcaaaagtga tcgcttccct atcagtgata gagacttcac gggataatgt
     7021 gttctactcc ctatcagtga tagagagctt aagtcaatcc aatgcgttca ggtgctccct
     7081 atcagtgata gagaaactcc gtcatcgtgg atgtcaatac tgactcccta tcagtgatag
     7141 agattgattt caccgctggc gtcaattccc tatcagtgat agagacttag gtgtccctag
     7201 aattactaga gatcgatttt agagctgcct ccctatcagt gatagagatt gctacaactg
     7261 caaggtagtc ccggtatccc tatcagtgat agagactgat agctacgagt ctgagatcct
     7321 acttgtccct atcagtgata gagacagtac cgagttttcc ctatcagtga tagagagctc
     7381 atactgcgac agagtctttt ccctatcagt gatagagata ggctcctttg tcaaaagtga
     7441 tcgcttccct atcagtgata gagacttcac gggataatgt gttctactcc ctatcagtga
     7501 tagagagctt aagtcaatcc aatgcgttca ggtgctccct atcagtgata gagaaactcc
     7561 gtcatcgtgg atgtcaatac tgactcccta tcagtgatag agattgattt caccgctggc
     7621 gtcaattccc tatcagtgat agagacttag gtgtccctag aattactaga gatcgatttt
     7681 agagctgcct ccctatcagt gatagagatt gctacaactg caaggtagtc ccggtatccc
     7741 tatcagtgat agagactgat agctacgagt ctgagatcct acttgtccct atcagtgata
     7801 gagacagtac cgagttttcc ctatcagtga tagagagctc atactgcgac agagtctttt
     7861 ccctatcagt gatagagata ggctcctttg tcaaaagtga tcgcttccct atcagtgata
     7921 gagacttcac gggataatgt gttctactcc ctatcagtga tagagagctt aagtcaatcc
     7981 aatgcgttca ggtgctccct atcagtgata gagaaactcc gtcatcgtgg atgtcaatac
     8041 tgactcccta tcagtgatag agattgattt caccgctggc gtcaattccc tatcagtgat
     8101 agagacttag gtgtccctag aattactaga gccaggcatc aaataaaacg aaaggctcag
     8161 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     8221 cacactggct caccttcggg tgggcctttc tgcgtttata tatgatgtgt aatgacaatt
     8281 atccttcccc aggaaaattg taatagccct gttgaacata taaatctagt ttagttattg
     8341 acgccaaata tacggtgttt aagtgaaata ttcaaactta ttttatattt atctgccggt
     8401 ttgttctatt ttgctgagtg ataggggaat attacccgga aactttccat atcattacaa
     8461 ataaagccct ctgtatgccc cggattttga atataatttg tatgtttaat ccgttttttg
     8521 tatctttgtg ccagcaagat actggaacag gctgttttta tacataaaaa tgctattttt
     8581 tgctattcgt tgaaaggatt atcttatcaa attctattcc ttttttaatt actcagtctg
     8641 tcaataatca ttttattaat tatttcggga ttcattgtta tttgtccctt atttaaatca
     8701 gaactttata cgatataaag aaagattcct gttatcgttt gttcttgtct tgctgtgtat
     8761 attattttaa taacctaaac cattttattg tatgtccaga ttagataaaa gtaaagtgat
     8821 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     8881 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     8941 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     9001 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     9061 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     9121 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     9181 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     9241 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     9301 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     9361 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaatggcc
     9421 acatcggttc ctgctagaag gcccatcctg acggatggcc ttttggtatt cgagcattac
     9481 ctcatacttt ggatttttgt tttgtttgca atggttaatc tattgttaaa atttaaagtt
     9541 tcacttgaac tttcaaataa tgttcttata tttgcagtgt ccctatcagt gatagagagt
     9601 aggatcacac aacatttaaa aaataacatt atgaaagcac tcgaaaagag attcaaaggt
     9661 aatattaaca ataatttatt ttcaatggtg agcaagggcg aggagctgtt caccggggtg
     9721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtccgcggc
     9781 gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg caccaccggc
     9841 aagctgcccg tgccctggcc caccctcgtg accaccttag gctacggcgt ggcctgcttc
     9901 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     9961 tacgtccagg agcgcaccat ctctttcaag gacgacggta cctacaagac ccgcgccgag
    10021 gtgaagttcg agggcgacac cctggtgaac cgcatcgtgc tgaagggcat cgacttcaag
    10081 gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca caaggtctat
    10141 atcacggccg acaagcagaa gaacggcatc aaggctaact tcaagacccg ccacaacgtt
    10201 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
    10261 cccgtgctgc tgcccgacaa ccactacctg agccatcagt ccaagctgag caaagacccc
    10321 aacgagaagc gcgatcacat ggtcctgaag gagagggtga ccgccgccgg gattacacat
    10381 gacatggacg agctgtacaa gtaatagcgt tcagctcgac tgtgagaagg ccatcctgac
    10441 ggatggcctt tttttttcgt tataactagt tgcgttccta gccgctacta gtgtaattgc
    10501 ctatcttcca gtgatggaac agcatttgtg cattggctgc aacaatcagc cttacttgtg
    10561 cctgttctat ttccgaaccg accgcttgta tgaatgcatc aaaattcgtt ttctctacgt
    10621 tggattcctt gttgctcata ttgtgatgat aatttctaca aatatagtca ttggtaacta
    10681 tctatgaaac tgtttgatac ttttatagtt gattaaactt gttcatggca tttgccttaa
    10741 tatcatccgc tatgtcaatg tagggtttca tagctttgta gtcgctgtgt cccgtccatt
    10801 tcatgaccac ctgtgccggg attccgagag ccagcgcatt gcagatgaat gtcctttttc
    10861 ctgcatgggt actgagcaaa gcgtatttgt gtgtgacttc atcaatacgt tcatttccct
    10921 tgtagtaggt ttcccgtaca ggctcgttga tttctgccag ttcgcccagc tctttcaggt
    10981 aatcgttcat cttctggttg ctgatgacgg gcagagccat gtaattctcg aaatggatgt
    11041 ccttgtattt gtccagtatg gctttgctgt atttgttcag ttcaatcgtc aggctgtcgg
    11101 cagtcttgac tgtggttatt tcgatgtggt cggacttcac atcgcttctt ttcagattgc
    11161 gaacatccga ataccgcaaa ctcgtaaagc agcagaacag gaaaacatca cgcacacgtt
    11221 ccaggtattg cttatccttg ggtatctggt agtctttcag cttgttcagt tcatcccaag
    11281 tcaggaagat tacttttttc gaggtggttt tcagtttcgg tttgaacgta tcgtatgcaa
    11341 tgttctgatg atgtcctttc ttgaagctcc agcgcaggaa ccatttgagg aatcccattt
    11401 gcttgccgat ggtgctgttt ctcatatcct tggtgtcacg caggaagttg acgtattcgt
    11461 tcaatccaaa ctcgttgaaa tagttgaacg ttgcatcctc cttgaactct ttgaggtggt
    11521 tcctcactgc tgcaaatttt tcataggtgg atgccgtcca gttattctgg ttaccgcact
    11581 cttttacaaa ctcatcgaac acctcccaaa agctgacagg ggcttcttcc ggctgttctt
    11641 cactggtgtc tttcattctc atgttgaaag cttccttcaa ctgttgggtc gttggcatga
    11701 cctcctgcac ctcaaattcc ttgaaaatat tctggatttc ggcatagtat ttcagcaagt
    11761 ccgtattgat ttcggctgca ctttgcttta gcttgttggt acatccgttc tttacccgct
    11821 gcttatctgc atcccatttg gctacgtcaa tccggtagcc cgttgtaaac tcgatacgtt
    11881 ggctggcaaa gatgacacgc atacggatgg gtacgttctc tacgattggc acaccgttct
    11941 ttttccggct ctccaatgca aaaatgatgt tgcgcttgat attcataatt gggtgcgttt
    12001 gaaattctac acccaaatat acacccaatt attgagatag caaaagacat ttagaaacat
    12061 ttacttttac tctatattgt aatttacact tgattatcag tcgtttgcag ttttatgata
    12121 ttctgtgaaa gtataagttc gagagcctgt ctctccgcaa aaaacgctga aaatcagcag
    12181 attgcaaaac aaacaccctg ttttacaccc aagaatgtaa agtcgggtgt ttttgtttta
    12241 tttaagataa tacaaccact acataataaa agagtagcga tattaaaaga atccgatgag
    12301 aaaagactaa tatttatcta tccattcagt ttgatttctc aggactttac atcgtcctga
    12361 aagtatttgt tggtaccggt accgaggacg cgacagctgc agacgcgtta tgagtaaact
    12421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12541 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
    12601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    12781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    12841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    12901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    12961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc atggccgcgg
    13321 gattaaaagt cggggattgg tgaacaaaaa ggtgtttctc tctttaagag aaatatcgtt
    13381 ttgctaaaca gttgatattg aggtatcatt ttatcgtaaa agacattttt gctcaacaat
    13441 tgcttgacgg aaatcaacaa attttagcat tttgtaaaaa agtcgctata taatttggtg
    13501 aattggagtt attttcatat ttttgcatcc cgaagagttt ctcttaaaga gagaaacatc
    13561 ttttgcatac cttttccgac cgaattttta tgtcgtaaag aggggctttg cagggggtgg
    13621 actcagaaag atgagaatag atgactattg tagttgaaac acatagaaag ttgctgatat
    13681 acagaccgat acgcatatcg ggatgaacca tgagtacgtt cttttctcaa aaaacataaa
    13741 tattcgaaaa gagatgcaat aaattaagga gaggttataa tgaacaaagt aaatataaaa
    13801 gatagtcaaa attttattac ttcaaaatat cacatagaaa aaataatgaa ttgcataagt
    13861 ttagatgaaa aagataacat ctttgaaata ggtgcaggga aaggtcattt tactgctgga
    13921 ttggtaaaga gatgtaattt tgtaacggcg atagaaattg attctaaatt atgtgaggta
    13981 actcgtaata agctcttaaa ttatcctaac tatcaaatag taaatgatga tatactgaaa
    14041 tttacatttc ctagccacaa tccatataaa atatttggca acatacctta caacataagc
    14101 acaaatataa ttcgaaaaat tgtttttgaa agttcagcca caataagtta tttaatagtg
    14161 gaatatggtt ttgctaaaat gttattagat acaaacagat cactagcatt gctgttaatg
    14221 gcagaggtag atatttctat attagcaaaa attcctaggt attatttcca tccaaaacct
    14281 aaagtggata gcacattaat tgtattaaaa agaaagccag caaaaatggc atttaaagag
    14341 agaaaaaaat atgaaacttt tgtaatgaaa tgggttaaca aagagtacga aaaactgttt
    14401 acaaaaaatc aatttaataa agctttaaaa catgcgagaa tatatgatat aaacaatatt
    14461 agtttcgaac aatttgtatc gctatttaat agttataaaa tatttaacgg ctaaaaacaa
    14521 taggccacat gcaactgtaa atgtttacgc gggtaccgac accgcggtgg aggggaattc
    14581 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc
    14641 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa
    14701 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
    14761 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
    14821 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
    14881 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
    14941 tatcaacagg ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat
    15001 caattccgtt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
    15061 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
    15121 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
    15181 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
    15241 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca
    15301 agccaaccag gaagggcagc ccacctatca cggaattgat ccccctcgaa ttggaattct
    15361 agcctggaat agggaagcac cacatcacc
//
