LOCUS       sequence-503110-       11902 bp    DNA     circular SYN 17-MAR-2026
DEFINITION  PBT0779+80xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11902)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 17, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB179
FEATURES             Location/Qualifiers
     source          1..11902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2863..2881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2910..2928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2959..2977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2991..3009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3079..3097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3121..3139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3169..3187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3218..3236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3260..3278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3323..3341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3370..3388
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3419..3437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3451..3469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3493..3511
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3539..3557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3581..3599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3629..3647
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3678..3696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3720..3738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3783..3801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3830..3848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3879..3897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3911..3929
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3953..3971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3999..4017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4041..4059
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4138..4156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4180..4198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4243..4261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4290..4308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4339..4357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4371..4389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4413..4431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4459..4477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4501..4519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4549..4567
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4598..4616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4640..4658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      4693..4764
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4780..4807
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             5305..5928
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5378..5397)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    6093..6111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(6198..6200,6201..6203,6204..6917)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(6243..6264)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      6938..6965
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(8945..9736,9737..9805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9568..9587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11097..11485
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            11656..11765
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     2881 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     2941 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     3001 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     3061 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     3121 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     3181 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     3241 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     3301 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     3361 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     3421 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     3481 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     3541 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     3601 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     3661 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     3721 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     3781 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     3841 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     3901 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     3961 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     4021 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     4081 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     4141 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     4201 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     4261 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     4321 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     4381 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     4441 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     4501 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     4561 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     4621 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     4681 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     4741 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     4801 ttctgcgttt atatatgatg gcagcatcca ttgcctgaaa aataaccaga accgagtagc
     4861 ggtcttgtgc ataaagcata ctttgcagtt ctgacagctt ttcgatattt tgggctaact
     4921 gttctttggc atcctgtttg gacatatcag cagtaaatga gggattgaaa tcggatacat
     4981 gatgttcctt ccccggtttg gctgtaagct cttttaagac ttcttttttc attgttgtat
     5041 aattaggttg ttataggtat ataacagttg aataaagaaa tcagttcttg ccaaattaag
     5101 agcgttgaat agtaattccc caattatgta tcaacagatt ctacataatt ccacactaat
     5161 tccacaatta tatttatatt gtctgctgtt tatatgtttg attatcagtt atatatggcg
     5221 gagtgaaacg tttggcatct gctttgctat atattaagta caagaaacaa tagttctatt
     5281 attcacactt aaaaaattac gattatgtcc agattagata aaagtaaagt gattaacagc
     5341 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     5401 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     5461 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     5521 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     5581 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     5641 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     5701 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     5761 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     5821 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     5881 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     5941 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     6001 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     6061 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     6121 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     6181 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     6301 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6361 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     6421 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6481 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     6541 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     6601 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     6661 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     6721 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6781 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     6841 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     6901 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     6961 cttttttttt cgttataact agttgcgttc ctagccgcta ctagtgtaat tgcctatctt
     7021 ccagtgatgg aacagcattt gtgcattggc tgcaacaatc agccttactt gtgcctgttc
     7081 tatttccgaa ccgaccgctt gtatgaatgc atcaaaattc gttttctcta cgttggattc
     7141 cttgttgctc atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga
     7201 aactgtttga tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc
     7261 cgctatgtca atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac
     7321 cacctgtgcc gggattccga gagccagcgc attgcagatg aatgtccttt ttcctgcatg
     7381 ggtactgagc aaagcgtatt tgtgtgtgac ttcatcaata cgttcatttc ccttgtagta
     7441 ggtttcccgt acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt
     7501 catcttctgg ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta
     7561 tttgtccagt atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt
     7621 gactgtggtt atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc
     7681 cgaataccgc aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta
     7741 ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa
     7801 gattactttt ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg
     7861 atgatgtcct ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc
     7921 gatggtgctg tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc
     7981 aaactcgttg aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac
     8041 tgctgcaaat ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac
     8101 aaactcatcg aacacctccc aaaagctgac aggggcttct tccggctgtt cttcactggt
     8161 gtctttcatt ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg
     8221 cacctcaaat tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt
     8281 gatttcggct gcactttgct ttagcttgtt ggtacatccg ttctttaccc gctgcttatc
     8341 tgcatcccat ttggctacgt caatccggta gcccgttgta aactcgatac gttggctggc
     8401 aaagatgaca cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg
     8461 gctctccaat gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc
     8521 tacacccaaa tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt
     8581 tactctatat tgtaatttac acttgattat cagtcgtttg cagttttatg atattctgtg
     8641 aaagtataag ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa
     8701 aacaaacacc ctgttttaca cccaagaatg taaagtcggg tgtttttgtt ttatttaaga
     8761 taatacaacc actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac
     8821 taatatttat ctatccattc agtttgattt ctcaggactt tacatcgtcc tgaaagtatt
     8881 tgttggtacc ggtaccgagg acgcgacagc tgcagacgcg ttatgagtaa acttggtctg
     8941 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9001 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9061 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     9121 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9181 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9241 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9301 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9361 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9421 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9481 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9541 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9601 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9661 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9721 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9781 cgacacggaa atgttgaata ctcatactct tcctttttca atcatggccg cgggattaaa
     9841 agtcggggat tggtgaacaa aaaggtgttt ctctctttaa gagaaatatc gttttgctaa
     9901 acagttgata ttgaggtatc attttatcgt aaaagacatt tttgctcaac aattgcttga
     9961 cggaaatcaa caaattttag cattttgtaa aaaagtcgct atataatttg gtgaattgga
    10021 gttattttca tatttttgca tcccgaagag tttctcttaa agagagaaac atcttttgca
    10081 taccttttcc gaccgaattt ttatgtcgta aagaggggct ttgcaggggg tggactcaga
    10141 aagatgagaa tagatgacta ttgtagttga aacacataga aagttgctga tatacagacc
    10201 gatacgcata tcgggatgaa ccatgagtac gttcttttct caaaaaacat aaatattcga
    10261 aaagagatgc aataaattaa ggagaggtta taatgaacaa agtaaatata aaagatagtc
    10321 aaaattttat tacttcaaaa tatcacatag aaaaaataat gaattgcata agtttagatg
    10381 aaaaagataa catctttgaa ataggtgcag ggaaaggtca ttttactgct ggattggtaa
    10441 agagatgtaa ttttgtaacg gcgatagaaa ttgattctaa attatgtgag gtaactcgta
    10501 ataagctctt aaattatcct aactatcaaa tagtaaatga tgatatactg aaatttacat
    10561 ttcctagcca caatccatat aaaatatttg gcaacatacc ttacaacata agcacaaata
    10621 taattcgaaa aattgttttt gaaagttcag ccacaataag ttatttaata gtggaatatg
    10681 gttttgctaa aatgttatta gatacaaaca gatcactagc attgctgtta atggcagagg
    10741 tagatatttc tatattagca aaaattccta ggtattattt ccatccaaaa cctaaagtgg
    10801 atagcacatt aattgtatta aaaagaaagc cagcaaaaat ggcatttaaa gagagaaaaa
    10861 aatatgaaac ttttgtaatg aaatgggtta acaaagagta cgaaaaactg tttacaaaaa
    10921 atcaatttaa taaagcttta aaacatgcga gaatatatga tataaacaat attagtttcg
    10981 aacaatttgt atcgctattt aatagttata aaatatttaa cggctaaaaa caataggcca
    11041 catgcaactg taaatgttta cgcgggtacc gacaccgcgg tggaggggaa ttcccatgtc
    11101 agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag
    11161 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
    11221 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
    11281 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
    11341 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
    11401 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
    11461 aggttgaact gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcaattcc
    11521 gttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
    11581 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
    11641 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
    11701 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
    11761 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
    11821 caggaagggc agcccaccta tcacggaatt gatccccctc gaattggaat tctagcctgg
    11881 aatagggaag caccacatca cc
//
