LOCUS       sequence-503274-        9379 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  Constructs used for LplA-catalyzed labeling in mammalian cells. LplA
            is expressed as a fusion with mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Cavanagh PE, Xue AG, Dai SA, Qiang A, Matsui T, Ting AY
  TITLE     Computational design of conformation-biasing mutations to alter 
            protein functions.
  JOURNAL   Science. 2026 Jan 8:eadv7953. doi: 10.1126/science.adv7953.
  PUBMED    41505504
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2026 Jan 8:eadv7953. doi: 10.1126/science.adv7953."
COMMENT     Sequence Label: His6-mCherry-Flag-LplA W37V
FEATURES             Location/Qualifiers
     source          1..9379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     375..394
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    679..800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(849..865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(849..865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(862..884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    873..889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(897..903,904..921,922..927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    942..963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1080..1097)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1251..1839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1331..1350)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2010..2801,2802..2870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2633..2652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2871..2975)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3050..3069)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3241..3620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3621..3824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3775..3795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3869..3888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3869..3887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3907..3916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3928..3945
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3958..4665
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4103..4121)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4371..4390
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4675..4698
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5738..6326
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5791..5811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6209..6220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6335..6456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6463..6670
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      7392..7401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7410..7910)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7473..7491)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        complement(7938..7956)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7939..7956)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7976..7993)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7982..8206
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8252..8680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8339..8358)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8549..8570
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8689..8709)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8694..9023
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8874..9009
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8936..8955
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(9090..9379,1..505)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9144..9163)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
       61 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
      121 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
      181 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
      241 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
      301 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
      361 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
      421 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
      481 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
      541 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
      601 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
      661 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      721 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      781 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
      841 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      961 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1021 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1141 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1681 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1981 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2041 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2101 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2161 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2221 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2281 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2341 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2401 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2461 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2521 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2581 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2641 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2701 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2761 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2821 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2881 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2941 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3001 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     3061 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     3121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     3181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     3241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     3301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     3541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     3601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     3661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     3721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     3781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
     3841 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
     3901 gcggccgcca ccatggtgcg gggttctcat catcatcatc atcatggtat ggctagcatg
     3961 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     4021 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     4081 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     4141 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     4201 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4261 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     4321 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     4381 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     4441 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     4501 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     4561 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     4621 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagaagga tccagactac
     4681 aaggatgacg acgataagat gagcaccctg agactgctga tcagcgacag ctacgacccc
     4741 tggttcaacc tggccgtgga ggagtgcatc ttcagacaga tgcccgccac ccagagagtg
     4801 ctgttcctgg tgagaaacgc cgacaccgtg gtgatcggca gagcccagaa cccctggaag
     4861 gagtgcaaca ccagaagaat ggaggaggac aacgtgagac tggccagaag aagcagcggc
     4921 ggcggcgccg tgttccacga cctgggcaac acctgcttca ccttcatggc cggcaagccc
     4981 gagtacgaca agaccatcag caccagcatc gtgctgaacg ccctgaacgc cctgggcgtg
     5041 agcgccgagg ccagcggcag aaacgacctg gtggtgaaga ccgtggaggg cgacagaaag
     5101 gtgagcggca gcgcctacag agagaccaag gacagaggct tccaccacgg caccctgctg
     5161 ctgaacgccg acctgagcag actggccaac tacctgaacc ccgacaagaa gaagctggcc
     5221 gccaagggca tcaccagcgt gagaagcaga gtgaccaacc tgaccgagct gctgcccggc
     5281 atcacccacg agcaggtgtg cgaggccatc accgaggcct tcttcgccca ctacggcgag
     5341 agagtggagg ccgagatcat cagccccaac aagacccccg acctgcccaa cttcgccgag
     5401 accttcgcca gacagagcag ctgggagtgg aacttcggcc aggcccccgc cttcagccac
     5461 ctgctggacg agagattcac ctggggcggc gtggagctgc acttcgacgt ggagaagggc
     5521 cacatcacca gagcccaggt gttcaccgac agcctgaacc ccgcccccct ggaggccctg
     5581 gccggcagac tgcagggctg cctgtacaga gccgacatgc tgcagcagga gtgcgaggcc
     5641 ctgctggtgg acttccccga gcaggagaag gagctgagag agctgagcgc ctggatggcc
     5701 ggcgccgtga gataagaatt ctgcagatat ctcgcgaaat caacctctgg attacaaaat
     5761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     6181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6241 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6301 gatctccctt tgggccgcct ccccgcttaa ttaaaacttg tttattgcag cttataatgg
     6361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6421 tagttgtggt ttgtccaaac tcatcaatgt atcttacaat tgtccccagc atgcctgcta
     6481 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     6541 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     6601 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     6661 gaaggcacag gcggccgcct atcaattaag tttatgcccc aacttacttg gaagatcgca
     6721 gtaccgggct accgcaactt cgtgttgttc aacataggtc tcattgttag cctcttttat
     6781 tctctccaac ctgtaatcga cataataaac acctggcatc ttcagatttt tcgcgggctt
     6841 tttactccga taggtggtct tggcattcgc aatcaaatga cttcctccca caagtttcag
     6901 tgccatgtcg ttcctacctt cgaggccgcc gtctgccggg tacaatgtct ccgtgaatgc
     6961 ttcccacccc aacgtcttct tctgcatgac tgggccattg ctggtaaagt ttactcccct
     7021 aatcttgacg ttatagatca aacaaccatc ttgcaagcta gtgtcttgtg tagcggtgag
     7081 cactcctccg tcctcatacg tagtcacgcg ctcccatgtg aaaccttcgg gaaaactctg
     7141 tttaaagaaa tccggtatgc cttgcgtgtg gttaataaaa gtcttggaac cgtacagaaa
     7201 ggatgtagcg agtatgtcaa aggcgaacgg cagaggacca ccctcgacaa cctttatccg
     7261 catggtctga gtgccctcgt agggcttgcc ttctccctca ctggtacatt tgaagtgatg
     7321 gttatctacc gtcccctcca tatacaattt catgtgcata ttctccttga tcagctcctc
     7381 gcccttagac accatggtgg cggtaccccc tggggagaga ggtcggtgat tcggtcaacg
     7441 agggagccga ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc
     7501 aggaacaggg cccacactac cgccccacac cccgcctccc gcaccgcccc ttcccggccg
     7561 ctgctctcgg cgcgccctgc tgagcagccg ctattggcca cagcccatcg cggtcggcgc
     7621 gctgccattg ctccctggcg ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc
     7681 gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt cccgacttag gggcggagca
     7741 ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga tccgggtgac gctgcgaacg
     7801 gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga accacgccca
     7861 gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ctcgagcatg
     7921 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     7981 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     8041 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     8101 ctgagtaggt gtcattctat tckggggggt ggggtggggc aggacagcaa gggggaggat
     8161 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8221 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     8281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     8701 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8761 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8821 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     8881 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8941 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     9001 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     9061 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     9121 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     9181 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     9241 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     9301 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     9361 ggctgctatt gggcgaagt
//
