LOCUS       sequence-503393-        6731 bp    DNA     circular SYN 19-MAR-2026
DEFINITION  Expression of FlagTurboNTD-TPRN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6731)
  TITLE     Trinkle Lab plasmids
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFlagTurboNTD(C1)-TPRN
FEATURES             Location/Qualifiers
     source          1..6731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             122..916
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             122..916
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(176..195)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     786..805
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1104..1123)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1148..1195
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1524..2112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2013..2032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2287..2590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2591..2794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2745..2765
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2826..2849
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5745..5866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5782..5801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5836..5855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5873..6328)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6010..6031)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6222..6241
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6355..6459
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(6461..6731,1..87)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6461..6652
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6484..6504)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(6669..6731,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
      121 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
      181 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
      241 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
      301 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
      361 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
      421 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
      481 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
      541 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
      601 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
      661 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
      721 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
      781 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
      841 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
      901 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
      961 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     1021 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     1081 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     1141 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     1201 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     1261 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     1321 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     1381 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1441 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1501 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1561 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1621 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1681 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1741 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1801 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1861 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1921 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1981 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2041 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2101 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2161 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2221 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2281 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2341 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2401 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2461 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2521 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2581 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2641 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2701 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2761 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
     2821 gcatggacta caaagacgat gacgacaagg gctctaaaga caatactgtg cctctgaagc
     2881 tgatcgctct cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg
     2941 gcatgtccag ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg
     3001 tgttcaccgt gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta
     3061 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gttgactcca
     3121 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     3181 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     3241 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     3301 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     3361 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     3421 tggccggaat aacatctcga gctcaagctt cgaattctat ggcagccctt ggaagacctg
     3481 gatccggacc cagagcagca gtaccagcct ggaaacgcga aatactcgaa aggaaaagag
     3541 ctaaactggc agccttgggt ggcggggccg gcccaggtgc agccgaacct gaacaaaggg
     3601 ttctcgcaga atcacttggt ccccttaggg aaaatccatt tatgttgctt gaagcggaac
     3661 gccgcagagg tggcggagcc gccggcgcac gccttttaga acgctataga agggttcccg
     3721 gagttcgggc attacgggct gattcagttc tgataattga aaccgtcccg ggatttcccc
     3781 ctgccccacc tgcacccggc gctgctcaaa ttagagcggc agaagtttta gtctatggag
     3841 cacccccagg gagagtttcc agactcctcg aacggtttga tccaccggct gcccctagac
     3901 gaagaggttc tccagaacga gcaaggccac cgcccccacc accaccaccg gctcccccta
     3961 gacccccacc agctgcacca tcaccacctg ctgctccagg cccacgaggc ggcggagctt
     4021 cacctggcgc aaggagatcc gattttctgc aaaagacagg gtccaatagt tttacagtgc
     4081 atccgagggg ccttcatcgg ggagctggtg caagattgtt atctaatggc catagcgcgc
     4141 cggaaccaag ggcaggtcca gctaatcggc tggccggatc tcccccgggc agcggccaat
     4201 ggaaacctaa agtcgaaagc ggagacccaa gtctgcatcc acccccttct ccaggaacac
     4261 catccgcaac ccccgctagc ccaccagcgt ccgcgacccc aagtcaaagg caatgtgtgt
     4321 cagcggcgac atccactaat gatagctttg aaattcgccc agcacctaaa cccgtgatgg
     4381 aaacaattcc actgggcgat ctgcaagcta gggccctcgc atcccttcgg gccaatagcc
     4441 ggaactcctt tatggtgatt ccaaaatcta aagctagcgg agccccacca ccggaaggtc
     4501 ggcaaagcgt agaactccct aaaggggatt tggggcctgc atctccaagt caagaactgg
     4561 gcagtcaacc cgtccccggt ggtgacggag ctccagcttt aggcaaatca ccgcttgaag
     4621 tggaagccca atgggctgtg gaagaaggag catgccctcg caccgcgaca gcattagcag
     4681 atagagcaat cagatggcaa agacccagta gccctcctcc atttctccca gccgccagcg
     4741 aagaggccga accagccgaa gggttaagag tgccagggct tgctaagaac tctcgcgagt
     4801 acgttagacc aggactcccc gtgactttta ttgacgaagt ggatagcgaa gaagcacctc
     4861 aggctgcgaa gctcccttat ctgccccatc ccgctcgacc gctccatcca gctcggccag
     4921 gatgtgtcgc tgaactgcaa cctaggggtt ccaatacttt tactgtcgtc cctaaacgta
     4981 aacctggtac cttacaagat caacatttct cccaagctaa tcgcgaaccc cgacctcgtg
     5041 aagcagaaga agaagaagca tcatgtctgc tcggtccaac actcaagaag agatatccaa
     5101 cagtacacga aattgaagtc ataggcgggt atcttgctct ccagaaatct tgtctgacaa
     5161 aagcaggatc aagtaggaag aagatgaaaa tcagctttaa tgataagtct ctccaaacta
     5221 ccttcgaata tccatctgaa tcatctttag aacaagaaga ggaagttgat caacaagaag
     5281 aggaagaaga agaagaagaa gaggaagagg aagaagagga aggttctggt tctgaagaaa
     5341 agccattcgc cctgtttctc ccaagggcta cattcgtcag ttctgttagg ccagaatcca
     5401 gtcgcttacc cgaaggctca tccggtctta gttcttatac tccaaaacat agcgtcgctt
     5461 ttagtaaatg gcaagaacaa gcccttgaac aagcgccccg ggaagctgaa ccgccacctg
     5521 ttgaagcaat gttgaccccg gcttcacaaa acgatttaag cgattttcgg tcagaacccg
     5581 ctctttactt ttaagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
     5641 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     5701 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     5761 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5821 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     5881 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5941 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6001 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6061 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6121 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6181 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6241 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6301 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6421 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     6481 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6541 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6661 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6721 taattttttt t
//
