LOCUS       sequence-503397-        7172 bp    DNA     circular SYN 19-MAR-2026
DEFINITION  Expression of FlagTurboNTD-NOM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7172)
  TITLE     Trinkle Lab plasmids
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFlagTurboNTD(C1)-NOM1
FEATURES             Location/Qualifiers
     source          1..7172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1222..1242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1303..1326
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1949..1958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4663..4784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4700..4719)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4754..4773
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4791..5246)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4928..4949)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5140..5159
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5273..5377
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5379..5736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5379..5570
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5402..5422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5587..5722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5771..6565
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5771..6565
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5825..5844)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6435..6454
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6753..6772)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6797..6844
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagca tggactacaa agacgatgac
     1321 gacaagggct ctaaagacaa tactgtgcct ctgaagctga tcgctctcct ggctaatggc
     1381 gagttccata gtggcgaaca gctgggagaa accctgggca tgtccagggc cgctatcaac
     1441 aagcacattc agactctgcg cgactggggc gtggacgtgt tcaccgtgcc cggaaagggc
     1501 tactctctgc ccgagcctat cccgctgctg aacgctaaac agattctggg acagctggac
     1561 ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga
     1621 atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga
     1681 agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg
     1741 cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca
     1801 gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat
     1861 ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac atctcgagct
     1921 caagcttcga attctgcagt cgacggtacc atggcggcgt ccaggagcgc gggagaggcc
     1981 ggcccgggcg gctcccaggg acgcgtggtc cgcatgaagc gcagaggcgg gcgcgggccg
     2041 cgccgcggtc ctgctggcgg tggggagaag gccctgaaga ggctgaagct agcggtggag
     2101 gagttcgtgc acgcgacttc ggaaggcgag gctcccgggg gttgcgaggg gcgcggcgcc
     2161 ccggtgagct ttcgcccggg agggagaaaa agccgtaagg aactgaggaa ggagaagcgg
     2221 cacctgcgga aagcacgccg gctgcagagg acggcgggcc ccgaacaggg tcccggcctg
     2281 ggaggccgaa gcggagccga agaagccagc ggtcaccggc aggacacgga ggagcgcgcc
     2341 cgcccagccc ctagtcggga cccctcgcct cccaggaagc cgcggccgtc ccgggtcaag
     2401 gccaaggcca cggccgccac cgcaaagacc agaccctccg cagccgccac cgccgctgcc
     2461 cggaaacggg cgcttttagc ggcgaacgag gaggaggacc gagagatccg aaagctggag
     2521 cgttgcctcg gtttgaacaa gcgcaaaaag aaggacggca gcagctccgt gccgctgagc
     2581 tttgcacgcg acggtcttga ctatattctg ggagccctgg agtctgggaa aaatagcggc
     2641 ttgtacgaca gcagtggtga ggaggaggaa gatgccggac agacactccc cgaaagtgac
     2701 ttagagagtg actcccagga cgaaagtgag gaggaggagg agggagacgt agaaaaggaa
     2761 aagaaggcgc aggaagcaga agcgcagagc gaggacgacg acgaggatac agaagaggaa
     2821 cagggggaag aaaaggaaaa gggagcgcag gagaaaagga gggggaagag agtccgtttt
     2881 gcagaagatg aagaaaagag tgaaaattcc tcggaggacg gtgacataac ggataagagt
     2941 ctttgtggaa gtggtgaaaa gtacatccca cctcatgtga ggcaagctga ggagacagtg
     3001 gacttcaaga aaaaggaaga actagaaagg ctgaagaaac atgtaaaagg tctacttaac
     3061 aggttgagtg aacccaacat ggcttccatc agtgggcagc tggaggaact gtacatggcc
     3121 cacagcagaa aggacatgaa tgacaccctg acctccgctc tcatgggtgc ctgcgtcact
     3181 gcctcggcca tgcccagcag actgatgatg gagcatgttc tcttagtcag catccttcac
     3241 cacacagttg gaatcgaggt cggtgcccac tttctggagg cagtggtgag gaagttcgat
     3301 gccatctata aatacggaag cgaagggaaa gagtgtgaca acctgttcac cgtcattgcc
     3361 catttataca acttccacgt ggtacagtct ctcctcatct tcgacatttt gaaaaaactg
     3421 attggaactt tcaccgaaaa agatattgaa ctgatcttgt taatgctgaa aaacgtgggt
     3481 ttttcattga ggaaagatga tgctttatca cttaaggaat tgatcactga agcccagacc
     3541 aaagccagcg gggcaggcag cgagtttcag gaccagacca ggattcggtt tatgctagag
     3601 acgatgttgg ccctgaagaa caatgacatg cgcaaaattc caggctatga ccccgagccc
     3661 gtggagaagc tgaggaaact gcagagagct ttggtccgca acgccggctc aggttctgag
     3721 acgcagcttc gcgtctcctg ggacagtgtc ttgagtgcgg agcagacggg tcgctggtgg
     3781 attgtggggt ccgcctggag tggggccccg atgatcgaca acagtcacca tacgcacctg
     3841 cagaagcagc ttgtggggac ggtcagttca aagatcctag aactcgcccg gaagcagagg
     3901 atgaacacag acatccggag aaacatattc tgcacaataa tgacaagtga agattttttg
     3961 gatgcttttg aaaagcttct gaagcttgga cttaaggatc agcaggagag agaaatcatt
     4021 cacgttctca tggattgctg ccttcaagag aaaacttaca atcccttcta tgctttcctg
     4081 gctagcaaat tctgtgaata tgaaaggaga tttcagatga ctttccagtt cagcatatgg
     4141 gacaaatttc gggacttgga aaacttgcca gctacgaatt tctctaattt ggttcatctg
     4201 gtggcccact tgttgaagac aaaatcgctt tccctttcca tcttaaaggt agttgaattc
     4261 agtgaattgg acaaacccag agtccgtttt ttacgaaaag tattaagtat cctattaatg
     4321 gaaacagaag ttgaagacct cagtttaatt ttcacaagag tatctgacaa cccaaagctg
     4381 ggggtgttac gtgagggttt gaagcttttc atcagccact tcttgctaaa gaacgcacag
     4441 gcccacagaa gcgccgacga agccaacgtg ctgagagaga aagctgacct tgcaacgaag
     4501 tgtctgcaag gaaaagcttc cctgagaatg tagggatcca ccggatctag ataactgatc
     4561 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4621 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4681 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4741 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     4801 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4861 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4921 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4981 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     5041 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     5101 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     5161 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     5221 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     5281 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5341 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     5401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5641 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5701 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5761 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5821 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5881 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5941 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6001 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6061 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6121 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6181 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6241 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     6301 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6361 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6421 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6481 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6541 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6601 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6661 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6721 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6781 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6841 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     6901 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     6961 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     7021 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7081 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7141 tcagaccccg tagaaaagat caaaggatct tc
//
