LOCUS       sequence-503666-        5621 bp    DNA     circular SYN 23-MAR-2026
DEFINITION  P12 driving Himar.004 codon harmonized transposase | Barcoded 
            Erythromycin Resistance Cassette Tn Payload | Conjugative Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ortiz L, Esmurria A, Gilbert C, Crits-Christoph A, Barnum TP, 
            Mancuso CP, Kang SC, Leung J, Fenn K, Abrams MB, Brumwell SL, Lee 
            HH, Ostrov N
  TITLE     A genetic platform for a biocementation bacterium
  JOURNAL   bioRxiv 2025.12.05.692644
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.12.05.692644"
COMMENT     Sequence Label: pGLU_446
FEATURES             Location/Qualifiers
     source          1..5621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2624..2640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2624..2640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2637..2659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      2830..3218
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(3351..3375)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3475..4266,4267..4335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4098..4117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4336..4440)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4539..4561
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4553..4570
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4554..4570
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            4703..4811
                     /note="incP origin of transfer"
ORIGIN
        1 cgcttgggcg agggttcacc cgtggccgtc cactccgccg actggcgatt agactccgga
       61 gtgtagtgat ggagccacgt ttcatccatc gttacatagc gacggaaaaa ctcaggcgta
      121 ttacgagtaa gcaactgtaa acacctcttc gaatcatcta cacggcgttg tttttggtca
      181 aacgtgagct ctctgggtac ccatttggca cagagcttcc tcatatctaa atattgatgg
      241 atgatatgac ctacacgttc cttagagatc ttaagtgcct ccgcgatctc gattaacttc
      301 attttacggt catttaagat cattttgtgg atttttttga tgttttcgtc cgttactacc
      361 tctttgggtc tgccagatct ttcaccgtcc tcggtcgaca tttcaccacg cttgaatttc
      421 gcataccaat cgatgatcgt agatttccca ggtgccgagt cgggaaactc attatctagc
      481 caagttttcg cttctaccgt atttttgccc ttcaaaaaac agtatttgat taatacacga
      541 aattcctttt tttccatatg taaagctcct ttcaaatcgg gactttagtg tctttagtaa
      601 ataagactca cttaaaagaa aagtatttcc taagtaaaca aaaatcccat tgccattata
      661 agtttattct atgataaagt acgatcgata actgtgctgt ttttaatctt aacaaagaca
      721 tcttcacaaa acaaattaat attttgaaaa atgccccttc tctctatcat aagcttttca
      781 agaagaagat gcaaaaacca aggttttgaa aagacaagat atgttatgtt tctttttgcc
      841 tggcagtcct atttccacta ctttattctt catcagacca catggacaaa ataataaaac
      901 aaaaaagacc gctttccctc ccaaaataaa gctttaggag gggaatgcat ggtcagtttc
      961 tttcaagttt ttttattcaa aggaccaaaa cgaaaaaagg cccccctttc gggaggcctc
     1021 ttttctggaa tttggtaccg agtaacaggt tggatgataa gtccccggtc taccagcttc
     1081 acacggcgtg tggctgcgga acacactcga agtaacaaaa tatgcaacga aaagcacatg
     1141 accaacatta ccctactcga cgcgtcctac gcgtgacccg atcctttaac tctggcaacc
     1201 ctcaaaattg aatgagacat gctacacctc cggataataa atatatataa acgtatatag
     1261 atttcataaa gtctaacaca ctagacttat ttacttcgta attaagtcgt taaaccgtgt
     1321 gctctacgac caaaactata aaacctttaa gaactttctt tttttacaag aaaaaagaaa
     1381 ttagataaat ctctcatatc ttttattcaa taatcgcatc cgattgcagt ataaatttaa
     1441 cgatcactca tcatgttcat atttatcaga ggtcgtgcta taattatact aattttataa
     1501 ggaggaaaaa atatgggcat ttttagtatt tttgtaatca gcacagttca ttatcaacca
     1561 aacaaaaaat aagtggttat aatgaatcgt taataagcaa aattcatata accaaattaa
     1621 agagggttat aaatgaacga gaaaaatata aaacacagtc aaaactttat tacttctaag
     1681 cataatatcg acaagattat gacaaacata agattaaatg aacatgataa tatctttgaa
     1741 atcggctcag gaaaaggcca ttttaccctt gaattagtaa agaggtgtaa tttcgtaact
     1801 gccattgaaa tagaccataa attatgcaaa actacagaaa ataaacttgt tgatcacgat
     1861 aatttccaag ttttaaacaa ggatatattg cagtttaaat ttcctaaaaa ccaatcctat
     1921 aaaatatatg gtaatatacc ttataacata agtacggata taatacgcaa aattgttttt
     1981 gatagtatag ctaatgagat ttatttaatc gtggaatacg ggtttgctaa aagattatta
     2041 aatacaaaac gctcattggc attactttta atggcagaag ttgatatttc tatattaagt
     2101 atggttccaa gagaatattt tcatcctaaa cctaaagtga atagctcact tatcagatta
     2161 agtagaaaaa aatcaagaat atcacacaaa gataaacaaa agtataatta tttcgttatg
     2221 aaatgggtta acaaagaata caagaaaata tttacaaaaa atcaatttaa caattcctta
     2281 aaacatgcag gaattgacga tttaaacaat attagctttg aacaattctt atctcttttc
     2341 aatagctata aattatttaa taagtaagct tgttaaggga tgcataaact gcatccctta
     2401 acttgttttt cgtgtgccta ttttttgtga atcgattatg tcttttgcgc agtcggctag
     2461 accggggact tatcatccaa cctgttacgc taaccccagg atacatagat taccacaact
     2521 ccgagccctt ccacctagcg tgctaaccac gcacgctatt gttgtatagg ctaggtggag
     2581 gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg
     2641 tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca
     2701 agacattagg tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc
     2761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt tacagctggg
     2821 cgcgccccat gtcagccgtt aagtgttcct gtgtcactca aaattgcttt gagaggctct
     2881 aagggcttct cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaagg
     2941 ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt
     3001 aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg
     3061 agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta
     3121 aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt
     3181 acgtactatc aacaggttga actgctgatc ttcagatcgg cgcgccggcc ggccgaagat
     3241 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     3301 cagcgtaaat gggtaggggg cttcaaatcg tcctcgtgat accaattcgg agcctgcttt
     3361 tttgtacaaa cttgttgata atggcaattc aaggatcttc acctagatcc ttttaaatta
     3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3601 tgcaatgata ccgcgagagc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4441 cacatttccc cgaaaagtgc cagatacctg aaacaaaacc catcgtacgg ccaaggaagt
     4501 ctccaataac tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac
     4561 gacggccagt catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac
     4621 cttagcgtgc gaacagcacg ctattgttgt atcattactc gcatccattc tcaggctgtc
     4681 tcgtctcgtc tccgaaggag tacggccagc ctcgcagagc aggattcccg ttgagcaccg
     4741 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     4801 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     4861 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     4921 cgaagcaggg ttatgcagcg gaaaagtcgt tgggattgtc ctgaccgaag cgggacaggt
     4981 ccacgagcag gcaatgcttg ttgggcatgg tgaggtggat gtctttcact tccggcacgc
     5041 agccgaggac tttttgcgcc atatcgtaaa gtgtgtgctg aacggattcg ctttcgtgtt
     5101 cagcgaaaga ggcgagaagg agattccaag cttattctac atagttccct tctagcgcga
     5161 tacaacgatt ataacgacct tctaattttt tgataccatt ttggtagtac tccttgggtt
     5221 tcgcctcaaa atatgcctca gtttctgcga ttacctcttc attgcaacca aattttttcc
     5281 ccgcgagcat ccgtttgagg tcagagaata agaaaaagtc cgaaggtgcc aaatcaggcg
     5341 aatagggggg gtgaggtagt aattcgaaac ctaattcatg gattttcgcc atggttcgta
     5401 aagacttgtg gcagggcgcg ttgtcttggt ggaataatac ttttttcttc ttcatgtgag
     5461 gccttttcgc ggcgatttcg acctttaaac gctctaaaag ggccatataa tagtcagagt
     5521 tgatcgtttt gcccttctct agataatcga taaagatgat gccatgggca tcccaaaata
     5581 ccgatgccat cactttaccc gcagactttt gagtcttgcc a
//
