LOCUS       sequence-503752-        6929 bp    DNA     circular SYN 23-MAR-2026
DEFINITION  Mammalian expression of RNA edited version of human 5-HT2C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Bonniwell EM, Alabdali R, Hennessey JJ, McKee JL, Cavalco NG, 
            Lammers JC, Moore EJ, Franchini L, Orlandi C, McCorvy JD
  TITLE     Serotonin 5-HT(2C) Receptor Signaling Analysis Reveals Psychedelic 
            Biased Agonism.
  JOURNAL   ACS Chem Neurosci. 2025 Oct 1;16(19):3899-3914. doi: 
            10.1021/acschemneuro.5c00647. Epub 2025 Sep 13.
  PUBMED    40944639
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschemneuro.5c00647"; journalName: "ACS Chem Neurosci"; 
            date: "2025-10-1- 1"; volume: "16"; issue: "19"; pages: "3899-3914"
COMMENT     Sequence Label: 5-HT2C_VSI
FEATURES             Location/Qualifiers
     source          1..6929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     265..284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(503..607)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(682..701)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        873..1252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1253..1456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1407..1427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1501..1520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1501..1519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1691..1717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3065..3106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3116..3133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3156..3173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3162..3386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3432..3860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3519..3538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3729..3750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3869..3889)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3874..4203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4054..4189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4116..4135
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4270..5064
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4324..4343)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4934..4953
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5240..5361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5277..5296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5331..5350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5410..5426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5410..5426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5423..5445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5434..5450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5458..5464,5465..5482,5483..5488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5503..5524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5641..5658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5812..6400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5892..5911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6571..6929,1..433,434..502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
       61 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
      121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
      181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
      241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
      301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
      361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      601 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
      661 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
      721 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
      781 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      841 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      901 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      961 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1021 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1081 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1141 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1201 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1261 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1321 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1381 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1441 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     1501 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
     1561 tccactagtc cagtgtggtg gaattgccct taccatggtg aacctgagga atgcggtgca
     1621 ttcattcctt gtgcacctaa ttggcctatt ggtttggcaa tgtgatattt ctgtgagccc
     1681 agtagcagct tacccatacg atgttccaga ttacgctata gtaactgaca ttttcaatac
     1741 ctccgatggt ggacgcttca aattcccaga cggggtacaa aactggccag cactttcaat
     1801 cgtcatcata ataatcatga caataggtgg caacatcctt gtgatcatgg cagtaagcat
     1861 ggaaaagaaa ctgcacaatg ccaccaatta cttcttaatg tccctagcca ttgctgatat
     1921 gctagtggga ctacttgtca tgcccctgtc tctcctggca atcctttatg attatgtctg
     1981 gccactacct agatatttgt gccccgtctg gatttcttta gatgttttat tttcaacagc
     2041 gtccatcatg cacctctgcg ctatatcgct ggatcggtat gtagcagtgc gtagtcctat
     2101 tgagcatagc cgtttcaatt cgcggactaa ggccatcatg aagattgcta ttgtttgggc
     2161 aatttctata ggtgtatcag ttcctatccc tgtgattgga ctgagggacg aagaaaaggt
     2221 gttcgtgaac aacacgacgt gcgtgctcaa cgacccaaat ttcgttctta ttgggtcctt
     2281 cgtagctttc ttcataccgc tgacgattat ggtgattacg tattgcctga ccatctacgt
     2341 tctgcgccga caagctttga tgttactgca cggccacacc gaggaaccgc ctggactaag
     2401 tctggatttc ctgaagtgct gcaagaggaa tacggccgag gaagagaact ctgcaaaccc
     2461 taaccaagac cagaacgcac gccgaagaaa gaagaaggag agacgtccta ggggcaccat
     2521 gcaggctatc aacaatgaaa gaaaagcttc gaaagtcctt gggattgttt tctttgtgtt
     2581 tctgatcatg tggtgcccat ttttcattac caatattctg tctgttcttt gtgagaagtc
     2641 ctgtaaccaa aagctcatgg aaaagcttct gaatgtgttt gtttggattg gctatgtttg
     2701 ttcaggaatc aatcctctgg tgtatactct gttcaacaaa atttaccgaa gggcattctc
     2761 caactatttg cgttgcaatt ataaggtaga gaaaaagcct cctgtcaggc agattccaag
     2821 agttgccgcc actgctttgt ctgggaggga gcttaatgtt aacatttatc ggcataccaa
     2881 tgaaccggtg atcgagaaag ccagtgacaa tgagcccggt atagagatgc aagttgagaa
     2941 tttagagtta ccagtaaatc cctccagtgt ggttagcgaa aggattagca gtgtgtaaaa
     3001 gggcaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     3061 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca
     3121 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3181 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3241 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3301 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3361 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3421 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3841 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4021 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4081 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4141 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4201 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4261 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4321 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4381 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4441 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4501 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4561 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4621 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4681 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4741 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4801 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4861 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4921 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4981 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5041 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     5101 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5161 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5221 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5341 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5401 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5461 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5521 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6241 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6901 gtcacgctcg tcgtttggta tggcttcat
//
