LOCUS       sequence-503770-        6997 bp    DNA     circular SYN 23-MAR-2026
DEFINITION  Expression vector of mouse Slc9c1(aa588-1175) tagged with PA at 
            C-terminus under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Iida-Norita R, Miyata H, Ninomiya A, Emori C, Kamoshita M, Pan C, 
            Wang H, Ikawa M
  TITLE     Formation of a complex between TMEM217 and the sodium-proton 
            exchanger SLC9C1 is crucial for mouse sperm motility and male 
            fertility.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2513924122. doi: 
            10.1073/pnas.2513924122. Epub 2025 Oct 15.
  PUBMED    41091759
REFERENCE   2  (bases 1 to 6997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-10-21"; volume: "122(42):e2513924122. 
            doi"; pages: " 10.1073/pnas.2513924122. Epub 2025 Oct 15"
COMMENT     Sequence Label: pCAG-mouse Slc9c1 (Delta-Exchanger)-PA
FEATURES             Location/Qualifiers
     source          1..6997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        398..674
                     /label=chicken beta-actin promoter
     intron          675..1689
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1615..1637
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1697..1716
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1780..1789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3678..3697)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3743..3798
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3797..3816)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4153..4172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4154..4172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4182..4199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4182..4198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4191..4213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4340..4795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4427..4446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4637..4658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4821..4925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4926..4994,4995..5786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5144..5163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5957..6544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6446..6465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6698..6715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6832..6853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6868..6873,6874..6891,6892..6898)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6906..6922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6911..6933
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6930..6946
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6930..6946
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6965..6985
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6967..6985
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggggg ggggccgggg ggggcggggc
      541 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
      601 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      661 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      721 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      781 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
      841 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
      901 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      961 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1021 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1081 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1141 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1201 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1261 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     1321 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1381 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1441 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1501 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1561 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1621 gcttctggcg tgtgaccggc ggctctagcc tctgctaacc atgttcatgc cttcttcttt
     1681 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaatct
     1741 agaggatccc tgcaggaatt cgatatcaag cttgtcgacg ccgccatgga aagcaaaaaa
     1801 ctcctctcct ttcttagaaa attactactc aactgggtgt ataatactaa aaaagataaa
     1861 ggggttccat caagatacat gtttcttcat gcatgccata gaatagtctt cacaaatgaa
     1921 tttgaatata ctggatacct tgtggtatta atgagcacat atcctatgat aatctgttgg
     1981 atttcccgac taaaagacat ctatgacaac gagataaagt gtgctaacta ctattttctt
     2041 gccttctata ttctagaggc tctacttaag gtggcagcaa tgaggaagga atttttttca
     2101 cacacctggc tcctgtttga gctggggatt accttagtcg gcgtcctaga tataatactt
     2161 atcgaaacag actccattag ttacaatttt gacttaactg agactgtggt cttcatgaac
     2221 gtgattcgac tccttcgtat actgcgcatc ttgaagcttg taacaccaaa actgctgcaa
     2281 atcatagaca aaaggatgag ccagcagata tcatttcggt attctatact gaaaggctat
     2341 gtccaagggg aaatggatgt actgaatata attgatcaga ttgcaagttc caaacagact
     2401 aaacagatat tgttaaagcg ggtaatgagg aatatggaac atgctatgaa agagctaggc
     2461 tacttagagt atgaccatcc ggaaatcgct gtcactatga aaaccaagga ggagattaat
     2521 gtcatgctca atatggccag agaaattgtc aaggctttca ggtccaaagg aattatacac
     2581 aaggtggaag gcactgagat taacaagtta atcatggcca aaaagatcca ggtgcttgat
     2641 ctgcagtctg ttatccagcc atttaatgtt gaagaagccc cgtgcaatat cccatggctt
     2701 agtgaagatc ctgaagccat aacctttatt caggaaaaag caaaagttgt aacatttgac
     2761 tgtggaaata acatatttga agaaggtgat gagccagaag gaatctatgt aataatttca
     2821 ggcatggtta agcttaaaag gtcaaaacca cacctggaga tggaaagagt atccgcagag
     2881 tcagagatta aaattcatcc actgccccac acagagtacc tgctcagcgg ggagataata
     2941 ggagagttaa actgtctgac taaagaacgg atgcaatatt ctgccacctg caaaactgtt
     3001 gtggagacat attttattcc cattagccac ttgtatgaag gctttgaaaa aagatgtcct
     3061 aacatgaaac ataaaatgtg gcaaaaaatc ggacttgcca ttactgccca aaagatccga
     3121 gaacacttat cttttgagga ctggaactac aagctgcagt tgaaactctg caatgccttc
     3181 ataagagaca tccccaagtc catgaaaact gacatctatg acgaaacggt aacccacgtt
     3241 gtcctcatcc atggatctgc tgaggactgc cagctgcgaa aaatttataa ggcgcctttc
     3301 ctaattcctg tgacgtgcca tcagatacaa ggcatggaag acttcacaaa agtgatgatt
     3361 attcaaactt caattgctgt aagaaaattc agatggaatg taagaaagta catcccacct
     3421 cgaagaattt cgatgaaacc agattctgaa agagaatctt ttgaaacatt ggatgaaacc
     3481 tctgaagagg acaatggtaa gaaagagaat caagaaaatg aggaattgat agaagagaat
     3541 attaacatcg ctagcggcgt tgccatgcca ggtgccgaag atgatgtggt gtaaatcgag
     3601 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     3661 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     3721 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     3781 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     3841 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     3901 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     3961 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     4021 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     4081 tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga cttaattaag
     4141 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4201 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4261 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4321 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4381 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4441 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4501 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4561 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4621 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4681 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4741 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5161 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6481 ttcgccacct ctgacttgag gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6601 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6661 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6721 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6781 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6841 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6901 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6961 gcgcgcaatt aaccctcact aaagggaaca aaagctg
//
