LOCUS       sequence-503848-        9914 bp    DNA     circular SYN 24-MAR-2026
DEFINITION  PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, strong 
            constitutive promoter CAG, insulated by cHS4 for random integration,
            puromycin resistance for selection in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Lebek T, Malaguti M, Boezio GL, Zoupi L, Briscoe J, Elfick A, Lowell
            S
  TITLE     PUFFFIN: an ultra-bright, customisable, single-plasmid system for 
            labelling cell neighbourhoods.
  JOURNAL   EMBO J. 2024 Jul 12. doi: 10.1038/s44318-024-00154-w.
  PUBMED    38997504
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 24, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2024 Jul 12. doi: 10.1038/s44318-024-00154-w."
COMMENT     Sequence Label: 
            pTL5_cHS4-CAG-s36GFPmNG-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4
FEATURES             Location/Qualifiers
     source          1..9914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1332..1635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1637..1914
                     /label=chicken beta-actin promoter
     intron          1915..2931
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2855..2877
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2939..2958
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3847..4554
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      4099..4108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4746..4763)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4973..4992
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5118..5714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5118..5137)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5614..5634
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5721..5777
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5784..6491
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5929..5947)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6197..6216
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6513..6533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6537..6557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6561..6581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     6691..6707
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6691..6707
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6716..6764
                     /note="synthetic polyadenylation signal"
     terminator      8190..8219
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      8241..8828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8729..8748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8840..8867)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(8891..9682,9683..9751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9514..9533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9752..9854)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(9855..9886)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aatggtcagt attgagcgat atctagagaa ttcgtatagg gcgttaatta actagagccc
       61 catcctcact gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg
      121 ggagggtcgg gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca
      181 cagctgccca cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat
      241 gtggcactga gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat
      301 cttggagggc ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt
      361 ctccagctgc ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca
      421 ccccttctgc ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag
      481 tgtctgtggc ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct
      541 atcctatctt cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag
      601 ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct
      661 gcctctccgt gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag
      721 caaactcccc cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg
      781 gttggatatg aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc
      841 aaggaggctg tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg
      901 ggcagcctgg gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc
      961 actgtgctcc tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca
     1021 gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     1081 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     1141 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     1201 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     1261 tctagggacg ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1321 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1381 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1441 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1501 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1561 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1621 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     1681 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     1741 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     1801 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     1861 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     1921 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     1981 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     2041 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     2101 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     2161 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     2221 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     2281 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     2341 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     2401 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     2461 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     2521 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     2581 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     2641 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     2701 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     2761 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     2821 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     2881 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     2941 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcctcga ctccgcctag
     3001 gcgcgccacc tcgaggccgc caccatgaag tgggtgacct tcatcagcct gctgtttctg
     3061 ttcagcagcg cctactctag agggggcgga gcaagcaaag gcgagaggct gtttagaggg
     3121 aaagtgccaa tcctggtgga actgaaagga gacgtgaacg gccacaagtt ctctgtccga
     3181 ggcaagggga aaggagatgc cacacgggga aaactgactc tgaagtttat ctgcaccaca
     3241 ggcaagctgc ccgtgccttg gccaaccctg gtcactaccc tgacatacgg cgtgcagtgt
     3301 ttcagccgat atccaaaaca catgaagcgg catgacttct ttaaaagtgc tatgcccaag
     3361 ggctacgtgc aggagcgcac catttcattc aagaaggatg ggaagtacaa gacccgggcc
     3421 gaggtgaagt ttgaagggag aaccctggtc aaccgcatca agctgaaagg acgcgacttc
     3481 aaggaaaaag ggaatattct gggacacaaa ctgagataca acttcaacag ccataaggtg
     3541 tacatcacag cagataagag aaaaaacggc atcaaggcca aattcaagat taggcacaat
     3601 gtgaaggacg gctccgtcca gctggctgat cattaccagc agaacacacc tattggcaga
     3661 gggcccgtgc tgctgcctag gaatcattat ctgagcactc gctccaaact gtctaaggac
     3721 cccaaagaga agcgggatca catggtgctg ctggaatttg tcaccgccgc tggcatcaaa
     3781 catgggcggg acgagagata caagggcggc agcggcggca ccagcggcgg cggcggcggc
     3841 agcggcgtga gcaagggcga ggaggataac atggcctctc tcccagcgac acatgagtta
     3901 cacatctttg gctccatcaa cggtgtggac tttgacatgg tgggtcaggg caccggcaat
     3961 ccaaatgatg gttatgagga gttaaacctg aagtccacca agggtgacct ccagttctcc
     4021 ccctggattc tggtccctca tatcgggtat ggcttccatc agtacctgcc ctaccctgac
     4081 gggatgtcgc ctttccaggc cgccatggta gatggctccg gataccaagt ccatcgcaca
     4141 atgcagtttg aagatggtgc ctcccttact gttaactacc gctacaccta cgagggaagc
     4201 cacatcaaag gagaggccca ggtgaagggg actggtttcc ctgctgacgg tcctgtgatg
     4261 accaactcgc tgaccgctgc ggactggtgc aggtcgaaga agacttaccc caacgacaaa
     4321 accatcatca gtacctttaa gtggagttac accactggaa atggcaagcg ctaccggagc
     4381 actgcgcgga ccacctacac ctttgccaag ccaatggcgg ctaactatct gaagaaccag
     4441 ccgatgtacg tgttccgtaa gacggagctc aagcactcca agaccgagct caacttcaag
     4501 gagtggcaaa aggcctttac cgatgtgatg ggcatggacg agctgtacaa gtaagcggcc
     4561 gcttgatatc gaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     4621 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4681 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4741 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4801 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4861 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4921 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4981 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     5041 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     5101 ggccccccga acccgtcatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     5161 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5221 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5281 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5341 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5401 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5461 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggtgtgtcg cccgaccacc
     5521 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5581 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5641 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5701 gcaagcccgg tgcctcactt gccacaaact tctctctgct aaagcaagca ggtgatgttg
     5761 aagaaaaccc cgggcctgcc tacatggtga gcaagggcga ggaggataac atggccatca
     5821 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     5881 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     5941 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     6001 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     6061 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     6121 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     6181 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     6241 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     6301 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     6361 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     6421 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     6481 agctgtacaa gacctcaggc aggacaggta gtccaaaaaa gaagagaaag gtagatccaa
     6541 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt attataagcg gccgctcgat
     6601 agcaaatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     6661 aaaggagcgc acgaccccat gcatcgatga tctagaacta gtggatcccc gatccaataa
     6721 aagatcttta ttttcattag atctgtgtgt tggttttttg tgtgggcttg ggggaggggg
     6781 aggccagaat gactccaaga gctacaggaa ggcaggtcag agaccccact ggacaaacag
     6841 tggctggact ctgcaccata acacacaatc aacaggggag tgagctggat ccggccctct
     6901 agagccccat cctcactgac tccgtcctgg agttggatga gagataatgg ccttacgttg
     6961 tgccagggga gggtcgggct ggatttagca agatttacct tctccaaaga gcggtgctgc
     7021 agtggcacag ctgcccacgg aggtgggggg gtcaccgtcc ctggaggtga tgaagaactg
     7081 tggggatgtg gcactgaggg acatggccag tgggcacggt gggtgggttg gggttggtct
     7141 tggggatctt ggagggcttt tccagccttc atgatttgac gattgtatga acatctacat
     7201 ggcaattctc cagctgcctg tcccagtcct actgacccag ctgtatctct ccaggcaagc
     7261 tcttccaccc cttctgcttg catccagaca ccatcaaaca tgcaggctca gacacaggga
     7321 ccagcagtgt ctgtggcctt tttgtgctcc tctccatgct gggttttaac ttgctctttg
     7381 tccttctatc ctatcttctt atccttaagg ctgttctgaa cgctgtgact tggagagtgt
     7441 cccagagccc tcaacacctg catgtcccac gtccatgctg tcctgcactt ccttatcccc
     7501 aagatctgcc tctccgtgat gcactgaatt ggcaaacatg tgtcacccca gaccaacaat
     7561 gtcacagcaa actccccctt gataggacaa gggggaatgg ctttacactg agacagggga
     7621 ggtttgggtt ggatatgagg aggcagtttt tcccccagag ggtggtgacg cactgaacag
     7681 gttgcccaag gaggctgtgg atgccccatc cctgcaggca ttcaaggcca ggctggatgt
     7741 ggctctgggc agcctgggct gctggttgat gaccctgcac atagcagggg gttggatctg
     7801 gatgagcact gtgctccttt gcaacccagg ccgttctatg attctgtcat tctaaatctc
     7861 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     7921 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     7981 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     8041 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
     8101 ctgtccctct agacgataac gaattcaagc ttgatatcat tcaggacgag cctcagactc
     8161 cagcgtaact ggactgcaat caactcactg gctcaccttc acgggtgggc ctttcttcgg
     8221 tagaaaatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8281 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct gccaactctt
     8341 tttccgaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8761 ggtttcgcca cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8821 tatggaaaaa cgccagcaac gcagaaaggc ccacccgaag gtgagccagg tgattacatt
     8881 tgggccctca ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8941 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatgcgg gagggcttac
     9001 catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat
     9061 cagcaataaa ccagccagcc gggagggccg agcgcagaag tgatcctgca actttatccg
     9121 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9181 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9241 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9301 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgc cagaagtaag ttggccgcag
     9361 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtga
     9421 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9481 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9541 taaaagtgct catcattgga aaacgttctt cggggcgtaa actctcaagg atcttaccgc
     9601 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9661 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9721 taagggcgac acggaaatgt tgaatactca ttttagcttc cttagctcct gaaaatctcg
     9781 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     9841 ttacgtgccg atcaagtcaa aagcctccgg tcggaggctt ttgactttct gctatggagg
     9901 tcaggtatga ttta
//
