LOCUS       sequence-503949-        7931 bp    DNA     circular SYN 24-MAR-2026
DEFINITION  Plasmid encoding FRT recombination site and TetOn3G transactivator 
            flanked by AAVS1 homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Norman D
  TITLE     Engineering and characterisation of AAVS1 FRT cell lines for 
            inducible and dose-dependent gene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 24, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAVS1 locus donor plasmid
FEATURES             Location/Qualifiers
     source          1..7931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     239..258
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        239..257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    272..1074
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     polyA_signal    1082..1163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1280..2026)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2045..2555)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2108..2126)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     polyA_signal    2575..2623
                     /note="synthetic polyadenylation signal"
     misc_feature    2637..2728
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2677..2696
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     primer_bind     complement(2740..2760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2758..3055
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2925..3059
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    3069..3116
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3127..3525
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3534..3615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3629..4465
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(4479..4497)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4480..4497)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4511..4528)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4511..4527)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4520..4542)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4668..5123)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4805..4826)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5017..5036
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5150..5254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5255..5323,5324..6115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5473..5492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(6250..6269)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6261..7076
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6261..7076
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6334..6353)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7161..7749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7650..7669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7903..7920
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
      241 atacgactca ctatagggaa agctcggtac ctgctttctc tgacctgcat tctctcccct
      301 gggcctgtgc cgctttctgt ctgcagcttg tggcctgggt cacctctacg gctggcccag
      361 atccttccct gccgcctcct tcaggttccg tcttcctcca ctccctcttc cccttgctct
      421 ctgctgtgtt gctgcccaag gatgctcttt ccggagcact tccttctcgg cgctgcacca
      481 cgtgatgtcc tctgagcgga tcctccccgt gtctgggtcc tctccgggca tctctcctcc
      541 ctcacccaac cccatgccgt cttcactcgc tgggttccct tttccttctc cttctggggc
      601 ctgtgccatc tctcgtttct taggatggcc ttctccgacg gatgtctccc ttgcgtcccg
      661 cctccccttc ttgtaggcct gcatcatcac cgtttttctg gacaacccca aagtaccccg
      721 tctccctggc tttagccacc tctccatcct cttgctttct ttgcctggac accccgttct
      781 cctgtggatt cgggtcacct ctcactcctt tcatttgggc agctccccta ccccccttac
      841 ctctctagtc tgtgctagct cttccagccc cctgtcatgg catcttccag gggtccgaga
      901 gctcagctag tcttcttcct ccaacccggg cccctatgtc cacttcagga cagcatgttt
      961 gctgcctcca gggatcctgt gtccccgagc tgggaccacc ttatattccc agggaaggtt
     1021 aatgtggctc tggttctggg tacttttatc tgtcccctcc acccccagtg gggcgtcgac
     1081 agcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1141 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1201 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     1261 attatgatcc tctggagatt tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt
     1321 cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc atgtctaagt
     1381 caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt ttctccaggc
     1441 cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca
     1501 gctcaattgc ttgtttcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct
     1561 cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg
     1621 cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct
     1681 gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc
     1741 cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt
     1801 gccatgactc gccttccagg gggcaggagt gggtatgatg cctgtccagc atctcgattg
     1861 gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa
     1921 ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgact ccattgagta
     1981 attccagagc agagtttatg actttgctct tgtccagtct agacatggta attcgatgat
     2041 ccccctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
     2101 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
     2161 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
     2221 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     2281 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     2341 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     2401 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     2461 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     2521 cagggctgcc ttggaaaagg cgcaacccca accccgtgga attatcacct cgagaataaa
     2581 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
     2641 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
     2701 gcaagtgcag gtgccagaac atttctctag atctgctgtg gaatgtgtgt cagttagggt
     2761 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2821 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2881 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2941 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3001 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggct
     3061 accatggaga agttactatt ccgaagttcc tattctctag aaagtatagg aacttcaagc
     3121 ttaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     3181 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     3241 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     3301 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     3361 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     3421 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     3481 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaatggtt gttaacttgt
     3541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3601 catttttttc actgcattct aggatatcac tagggacagg attaatgaca gaaaagcccc
     3661 atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca cctcctgtta
     3721 ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc tccttgccag
     3781 aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag agcttggcag
     3841 ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct
     3901 accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc actgatcctg
     3961 gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa
     4021 taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa tttatattgt
     4081 tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg tcctggcagg
     4141 gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct
     4201 aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc cttctttcct
     4261 taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg tcccgcttcc
     4321 ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga gaggcgctca
     4381 ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc tgccccttcc
     4441 ctacaggggt tcctggctct gctctgcggc cgctgtattc tatagtgtca cctaaatggc
     4501 cgcacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4561 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4621 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
     4681 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4741 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4801 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4861 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4921 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4981 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     5041 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     5101 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5161 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5221 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5281 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5341 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5401 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5461 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5521 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5581 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5641 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5701 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5761 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5821 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5881 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5941 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6001 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6061 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6121 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6181 gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct gcttacataa
     6241 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc
     6301 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     6361 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     6421 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     6481 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     6541 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     6601 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     6661 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     6721 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     6781 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     6841 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     6901 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     6961 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     7021 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat
     7081 taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7801 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7861 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7921 gaagcggaag a
//
