LOCUS       sequence-50405-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre activity reporter plasmid; Zsgreen is expressed in cells without
            Cre, mCherry is expressed with Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Yang YS, Hughes TE
  TITLE     Cre stoplight: a red/green fluorescent reporter of Cre recombinase 
            expression in living cells.
  JOURNAL   Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.
  PUBMED    11730010
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2001-11"; volume: "31(5)"; pages: "1036, 
            1038, 1040-1"
COMMENT     Sequence Label: Cre Stoplight 2.4
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(92..125)
                     /label=loxH
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021). The loxH variant 
                     was optimized for expression constructs but remains 
                     compatible with loxP."
     CDS             139..828
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSAL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag aattacctca tatagcatac attatacgaa
      121 gttatgggcg cgctcgagat ggcccagtcc aagcacggcc tgaccaagga gatgaccatg
      181 aagtaccgca tggagggctg cgtggacggc cacaagttcg tgatcaccgg cgagggcatc
      241 ggctacccct tcaagggcaa gcaggccatc aacctgtgcg tggtggaggg cggccccttg
      301 cccttcgccg aggacatctt gtccgccgcc ttcatgtacg gcaaccgcgt gttcaccgag
      361 tacccccagg acatcgtcga ctacttcaag aactcctgcc ccgccggcta cacctgggac
      421 cgctccttcc tgttcgagga cggcgccgtg tgcatctgca acgccgacat caccgtgagc
      481 gtggaggaga actgcatgta ccacgagtcc aagttctacg gcgtgaactt ccccgccgac
      541 ggccccgtga tgaagaagat gaccgacaac tgggagccct cctgcgagaa gatcatcccc
      601 gtgcccaagc agggcatctt gaagggcgac gtgagcatgt acctgctgct gaaggacggt
      661 ggccgcttgc gctgccagtt cgacaccgtg tacaaggcca agtccgtgcc ccgcaagatg
      721 cccgactggc acttcatcca gcacaagctg acccgcgagg accgcagcga cgccaagaac
      781 cagaagtggc acctgaccga gcacgccatc gcctccggct ccgccttg
//
