LOCUS       sequence-50407-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a 2A peptide construct that produces two different proteins 
            from a single open reading frame, a nuclear targeted Cre 
            recombinase, and a membrane targeted GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     Cre Shine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Cre Shine
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg
      121 gatccactag tccagtgtgg tggaattcac catgtccaat ttactgaccg tacaccaaaa
      181 tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat
      241 gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg
      301 gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga
      361 tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca
      421 acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag
      481 caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg
      541 tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga
      601 aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac
      661 cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg
      721 tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga
      781 gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt
      841 agctgatgat ccgaataact ac
//
