LOCUS       sequence-504082-        9618 bp    DNA     circular SYN 25-MAR-2026
DEFINITION  160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for 
            Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9618)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEM352
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    345..363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    436..454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    499..517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    546..564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    595..613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    627..645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    669..687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    715..733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    757..775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    805..823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    854..872
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    896..914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    959..977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1006..1024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1055..1073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1087..1105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1129..1147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1175..1193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1217..1235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1265..1283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1314..1332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1356..1374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1419..1437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1466..1484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1515..1533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1547..1565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1589..1607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1635..1653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1677..1695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1725..1743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1774..1792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1816..1834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1879..1897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1926..1944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1975..1993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2007..2025
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2049..2067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2137..2155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2185..2203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2234..2252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2276..2294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2364..2382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2412..2430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2503..2521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2566..2584
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2662..2680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2694..2712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2782..2800
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2824..2842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2872..2890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2921..2939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2963..2981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3026..3044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3073..3091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3122..3140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3154..3172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3196..3214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3242..3260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3284..3302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3332..3350
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3381..3399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3423..3441
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3486..3504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3533..3551
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3582..3600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3614..3632
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3702..3720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3744..3762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3792..3810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3841..3859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3883..3901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3946..3964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3993..4011
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4042..4060
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4074..4092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4116..4134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4162..4180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4204..4222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4252..4270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4301..4319
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4343..4361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4406..4424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4453..4471
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4502..4520
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4534..4552
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4576..4594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4622..4640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4664..4682
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4712..4730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4761..4779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4803..4821
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4866..4884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4913..4931
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4962..4980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4994..5012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5036..5054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5082..5100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5124..5142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5172..5190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5221..5239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5263..5281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5326..5344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5373..5391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5422..5440
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5454..5472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5496..5514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5542..5560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5584..5602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5632..5650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5681..5699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5723..5741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5786..5804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5833..5851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5882..5900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5914..5932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5956..5974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6002..6020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6044..6062
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6092..6110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6141..6159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6183..6201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6246..6264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6293..6311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6342..6360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6374..6392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6416..6434
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6462..6480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6504..6522
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6552..6570
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6601..6619
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6643..6661
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6706..6724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6753..6771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6802..6820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6834..6852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6876..6894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6922..6940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6964..6982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7012..7030
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7061..7079
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7103..7121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      7156..7227
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7243..7270
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7358..8173)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7358..8173)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8081..8100
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8165..8184
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(8634..8651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8768..9312)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8847..8866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgagcggcc gcttctagag atcgatttta gagctgcctc cctatcagtg atagagattg
       61 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
      121 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
      181 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
      241 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
      301 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
      361 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
      421 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
      481 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
      541 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
      601 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
      661 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
      721 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
      781 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
      841 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
      901 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
      961 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     1021 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     1081 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     1141 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     1201 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     1261 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     1321 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     1381 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     1441 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     1501 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     1561 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     1621 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     1681 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     1741 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     1801 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     1861 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
     1921 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
     1981 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
     2041 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
     2101 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
     2161 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
     2221 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
     2281 atcagtgata gagataggct cctttgtcaa aagtgatcgc ttccctatca gtgatagaga
     2341 cttcacggga taatgtgttc tactccctat cagtgataga gagcttaagt caatccaatg
     2401 cgttcaggtg ctccctatca gtgatagaga aactccgtca tcgtggatgt caatactgac
     2461 tccctatcag tgatagagat tgatttcacc gctggcgtca attccctatc agtgatagag
     2521 acttaggtgt ccctagaatt actagagatc gattttagag ctgcctccct atcagtgata
     2581 gagattgcta caactgcaag gtagtcccgg tatccctatc agtgatagag actgatagct
     2641 acgagtctga gatcctactt gtccctatca gtgatagaga cagtaccgag ttttccctat
     2701 cagtgataga gagctcatac tgcgacagag tcttttccct atcagtgata gagataggct
     2761 cctttgtcaa aagtgatcgc ttccctatca gtgatagaga cttcacggga taatgtgttc
     2821 tactccctat cagtgataga gagcttaagt caatccaatg cgttcaggtg ctccctatca
     2881 gtgatagaga aactccgtca tcgtggatgt caatactgac tccctatcag tgatagagat
     2941 tgatttcacc gctggcgtca attccctatc agtgatagag acttaggtgt ccctagaatt
     3001 actagagatc gattttagag ctgcctccct atcagtgata gagattgcta caactgcaag
     3061 gtagtcccgg tatccctatc agtgatagag actgatagct acgagtctga gatcctactt
     3121 gtccctatca gtgatagaga cagtaccgag ttttccctat cagtgataga gagctcatac
     3181 tgcgacagag tcttttccct atcagtgata gagataggct cctttgtcaa aagtgatcgc
     3241 ttccctatca gtgatagaga cttcacggga taatgtgttc tactccctat cagtgataga
     3301 gagcttaagt caatccaatg cgttcaggtg ctccctatca gtgatagaga aactccgtca
     3361 tcgtggatgt caatactgac tccctatcag tgatagagat tgatttcacc gctggcgtca
     3421 attccctatc agtgatagag acttaggtgt ccctagaatt actagagatc gattttagag
     3481 ctgcctccct atcagtgata gagattgcta caactgcaag gtagtcccgg tatccctatc
     3541 agtgatagag actgatagct acgagtctga gatcctactt gtccctatca gtgatagaga
     3601 cagtaccgag ttttccctat cagtgataga gagctcatac tgcgacagag tcttttccct
     3661 atcagtgata gagataggct cctttgtcaa aagtgatcgc ttccctatca gtgatagaga
     3721 cttcacggga taatgtgttc tactccctat cagtgataga gagcttaagt caatccaatg
     3781 cgttcaggtg ctccctatca gtgatagaga aactccgtca tcgtggatgt caatactgac
     3841 tccctatcag tgatagagat tgatttcacc gctggcgtca attccctatc agtgatagag
     3901 acttaggtgt ccctagaatt actagagatc gattttagag ctgcctccct atcagtgata
     3961 gagattgcta caactgcaag gtagtcccgg tatccctatc agtgatagag actgatagct
     4021 acgagtctga gatcctactt gtccctatca gtgatagaga cagtaccgag ttttccctat
     4081 cagtgataga gagctcatac tgcgacagag tcttttccct atcagtgata gagataggct
     4141 cctttgtcaa aagtgatcgc ttccctatca gtgatagaga cttcacggga taatgtgttc
     4201 tactccctat cagtgataga gagcttaagt caatccaatg cgttcaggtg ctccctatca
     4261 gtgatagaga aactccgtca tcgtggatgt caatactgac tccctatcag tgatagagat
     4321 tgatttcacc gctggcgtca attccctatc agtgatagag acttaggtgt ccctagaatt
     4381 actagagatc gattttagag ctgcctccct atcagtgata gagattgcta caactgcaag
     4441 gtagtcccgg tatccctatc agtgatagag actgatagct acgagtctga gatcctactt
     4501 gtccctatca gtgatagaga cagtaccgag ttttccctat cagtgataga gagctcatac
     4561 tgcgacagag tcttttccct atcagtgata gagataggct cctttgtcaa aagtgatcgc
     4621 ttccctatca gtgatagaga cttcacggga taatgtgttc tactccctat cagtgataga
     4681 gagcttaagt caatccaatg cgttcaggtg ctccctatca gtgatagaga aactccgtca
     4741 tcgtggatgt caatactgac tccctatcag tgatagagat tgatttcacc gctggcgtca
     4801 attccctatc agtgatagag acttaggtgt ccctagaatt actagagatc gattttagag
     4861 ctgcctccct atcagtgata gagattgcta caactgcaag gtagtcccgg tatccctatc
     4921 agtgatagag actgatagct acgagtctga gatcctactt gtccctatca gtgatagaga
     4981 cagtaccgag ttttccctat cagtgataga gagctcatac tgcgacagag tcttttccct
     5041 atcagtgata gagataggct cctttgtcaa aagtgatcgc ttccctatca gtgatagaga
     5101 cttcacggga taatgtgttc tactccctat cagtgataga gagcttaagt caatccaatg
     5161 cgttcaggtg ctccctatca gtgatagaga aactccgtca tcgtggatgt caatactgac
     5221 tccctatcag tgatagagat tgatttcacc gctggcgtca attccctatc agtgatagag
     5281 acttaggtgt ccctagaatt actagagatc gattttagag ctgcctccct atcagtgata
     5341 gagattgcta caactgcaag gtagtcccgg tatccctatc agtgatagag actgatagct
     5401 acgagtctga gatcctactt gtccctatca gtgatagaga cagtaccgag ttttccctat
     5461 cagtgataga gagctcatac tgcgacagag tcttttccct atcagtgata gagataggct
     5521 cctttgtcaa aagtgatcgc ttccctatca gtgatagaga cttcacggga taatgtgttc
     5581 tactccctat cagtgataga gagcttaagt caatccaatg cgttcaggtg ctccctatca
     5641 gtgatagaga aactccgtca tcgtggatgt caatactgac tccctatcag tgatagagat
     5701 tgatttcacc gctggcgtca attccctatc agtgatagag acttaggtgt ccctagaatt
     5761 actagagatc gattttagag ctgcctccct atcagtgata gagattgcta caactgcaag
     5821 gtagtcccgg tatccctatc agtgatagag actgatagct acgagtctga gatcctactt
     5881 gtccctatca gtgatagaga cagtaccgag ttttccctat cagtgataga gagctcatac
     5941 tgcgacagag tcttttccct atcagtgata gagataggct cctttgtcaa aagtgatcgc
     6001 ttccctatca gtgatagaga cttcacggga taatgtgttc tactccctat cagtgataga
     6061 gagcttaagt caatccaatg cgttcaggtg ctccctatca gtgatagaga aactccgtca
     6121 tcgtggatgt caatactgac tccctatcag tgatagagat tgatttcacc gctggcgtca
     6181 attccctatc agtgatagag acttaggtgt ccctagaatt actagagatc gattttagag
     6241 ctgcctccct atcagtgata gagattgcta caactgcaag gtagtcccgg tatccctatc
     6301 agtgatagag actgatagct acgagtctga gatcctactt gtccctatca gtgatagaga
     6361 cagtaccgag ttttccctat cagtgataga gagctcatac tgcgacagag tcttttccct
     6421 atcagtgata gagataggct cctttgtcaa aagtgatcgc ttccctatca gtgatagaga
     6481 cttcacggga taatgtgttc tactccctat cagtgataga gagcttaagt caatccaatg
     6541 cgttcaggtg ctccctatca gtgatagaga aactccgtca tcgtggatgt caatactgac
     6601 tccctatcag tgatagagat tgatttcacc gctggcgtca attccctatc agtgatagag
     6661 acttaggtgt ccctagaatt actagagatc gattttagag ctgcctccct atcagtgata
     6721 gagattgcta caactgcaag gtagtcccgg tatccctatc agtgatagag actgatagct
     6781 acgagtctga gatcctactt gtccctatca gtgatagaga cagtaccgag ttttccctat
     6841 cagtgataga gagctcatac tgcgacagag tcttttccct atcagtgata gagataggct
     6901 cctttgtcaa aagtgatcgc ttccctatca gtgatagaga cttcacggga taatgtgttc
     6961 tactccctat cagtgataga gagcttaagt caatccaatg cgttcaggtg ctccctatca
     7021 gtgatagaga aactccgtca tcgtggatgt caatactgac tccctatcag tgatagagat
     7081 tgatttcacc gctggcgtca attccctatc agtgatagag acttaggtgt ccctagaatt
     7141 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     7201 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     7261 cctttctgcg tttatatatg atcgagaccg gtaatctcga gtcccgtcaa gtcagcgtaa
     7321 tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
     7381 aatgaaactg caatttattc atatcaggat tatcgatacc atatttttga aaaagccgtt
     7441 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     7501 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     7561 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     7621 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     7681 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     7741 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     7801 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     7861 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     7921 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     7981 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     8041 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     8101 catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac gtttcccgtt
     8161 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     8221 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     8281 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
     8341 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
     8401 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct
     8461 ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgctagcgg agtgtatact
     8521 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     8581 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     8641 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     8701 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     8761 agccgttttt ccataggctc cgccccctga caagcatcac gaaatctgac gctcaaatca
     8821 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     8881 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     8941 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     9001 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     9061 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     9121 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     9181 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     9241 aaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     9301 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaactca
     9361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9421 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9481 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9541 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9601 gctgcaatga tggtctcg
//
