LOCUS       sequence-504090-        8019 bp    DNA     circular SYN 25-MAR-2026
DEFINITION  IPO4 tagged on the N-terminus with mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ
  TITLE     Evidence for the nuclear import of histones H3.1 and H4 as monomers.
  JOURNAL   EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub
            2018 Sep 3.
  PUBMED    30177573
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 25, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-10-1"; volume: "37(19):e98714. doi"; pages: " 
            10.15252/embj.201798714. Epub 2018 Sep 3"
COMMENT     Sequence Label: pCL0965
FEATURES             Location/Qualifiers
     source          1..8019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(758..776)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1026..1045
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1381..1401
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    4807..4928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4844..4863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4898..4917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4935..5390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5072..5093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5284..5303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5417..5521
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5523..5880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5523..5714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5546..5566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5731..5866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5915..6709
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5915..6709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5969..5988)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6579..6598
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6897..6916)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6941..6988
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7317..7905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7806..7825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cggcagcggc
     1381 gaaaaccttt acttccaggg ccatatgctc gagtttttca gcaagatgga gtcagccggg
     1441 ctagagcagc tcctacggga gctgctgcta ccggacaccg agcgcatccg tcgggccacg
     1501 gaacagctcc agatcgttct tcgggccccc gccgctttgc cggctctctg cgacctgcta
     1561 gcctcggcgg ccgaccccca gatccgccag tttgcggccg tgctgacccg cagacgactg
     1621 aacacccgct ggcgacggct ggcggcggag caacgggaga gcctcaagtc cctgatcctg
     1681 acggccctgc agagagaaac agagcactgt gtgagcctca gcctggccca gctctcagcc
     1741 accatttttc gaaaggaagg cttggaggcc tggccacagc ttttgcagct gcttcagcac
     1801 agtacccaca gcccccacag cccagagaga gagatggggc ttttgctgct aagtgtggtg
     1861 gtgacctccc ggcccgaggc cttccaaccc caccaccggg agcttcttcg gcttctgaat
     1921 gagactcttg gtgaggtggg ctctcctggg ctgctcttct actccctgcg cactctgacc
     1981 accatggctc cctacctcag cactgaagat gtgcctctcg ctcggatgtt ggtgcccaag
     2041 ctgatcatgg ccatgcagac tctgatcccc atagatgagg caaaggcctg tgaggccctt
     2101 gaggctttgg atgaactgtt ggagtcagag gtgccggtca tcacccccta cctctctgaa
     2161 gtcctcacat tctgcctgga ggtagctaga aatgtggccc tgggcaatgc gatacgcata
     2221 cgtattctct gctgcctcac tttcttggtc aaagtcaaga gcaaggcctt actgaagaat
     2281 cgtctcctgc cacccttgct gcacaccctt ttccccattg tggctgctga gcccccacca
     2341 ggccagttgg atcccgagga ccaggattca gaagaggaag agttggagat tgagctgatg
     2401 ggggagactc ccaagcattt cgctgtacaa gttgtggaca tgctggcact acacctgccc
     2461 cccgagaagc tctgtcccca gctgatgccc atgttggaag aggctttgcg gagcgagagc
     2521 ccataccagc gcaaagctgg actcctggtg ctggccgtgc tgtctgacgg agctggcgac
     2581 cacatcaggc agagactgct gcccccactg ctgcagattg tgtgcaaggg cctggaggac
     2641 ccctcgcaag ttgtacgcaa tgctgcgctg tttgccctgg gccagttctc agaaaaccta
     2701 cagccccata tcagcagcta ttcaagggag gtgatgccac tgctcctcgc ctacttgaag
     2761 tcggtgcctc ttggacacac acaccaccta gccaaggcct gctatgccct ggagaatttt
     2821 gtggagaacc tagggcccaa ggtgcagccc taccttccgg agcttatgga atgcatgctg
     2881 cagcttctga ggaaccccag cagtccccgg gccaaggagc tggctgtgag cgccctggga
     2941 gccattgcta cggctgccca ggtctcgctg ctgccctact tccctgccat catggagcac
     3001 ctgcgggaat tcctgttaac aggccgtgag gaccttcagc ctgtgcagat ccagagcctg
     3061 gagacactgg gggtgctggc acgagcagtg ggggagccca tgaggccgct ggctgaggaa
     3121 tgctgccagc tgggtctggg cctctgcgac caggtagacg acgctgactt gcggcgctgc
     3181 acgtacagcc tatttgcagc cttatcgggt ctgatgggtg agggcctggc gccccacttg
     3241 gaacagatca ccacgctcat gctgctgtca ctgcgttcca ccgagggcat tgtgcctcag
     3301 tatgacggga gcagctcctt ccttctgttt gacgatgaga gtgatgggga agaagaggag
     3361 gagctcatgg atgaggatgt ggaagaagag gatgactcag agatctcagg gtacagcgtg
     3421 gagaatgcct tcttcgatga gaaggaagac acctgtgctg ccgtggggga gatctctgtg
     3481 aacaccagtg tggccttcct tccatacatg gaaagtgtct ttgaagaagt atttaaactg
     3541 ctggagtgcc ctcacctgaa tgtgcggaag gcagcccatg aggctctggg tcagttttgc
     3601 tgtgcactgc acaaggcctg tcaaagctgc ccctcggaac ccaacactgc tgctttgcag
     3661 gctgccctgg cccgagtcgt gccatcctac atgcaggcag tgaacaggga gcgggaacgc
     3721 caggtggtga tggccgtgct ggaggccctg acaggggtgc tccgcagctg tgggaccctc
     3781 acactgaagc cccctgggcg cctcgctgag ctctgtggcg tgctcaaggc tgtgctgcag
     3841 aggaagacag cctgtcagga tactgacgag gaggaggaag aggaagatga tgatcaggct
     3901 gaatacgacg ccatgttgct ggagcacgct ggagaggcca tccctgccct ggcagccgcg
     3961 gctgggggag actcctttgc cccattcttt gccggtttcc tgccattatt ggtgtgcaag
     4021 acaaaacagg gctgcacagt ggcagagaag tcctttgcag tggggacctt ggcagagact
     4081 attcagggcc tgggtgctgc ctcagcccag tttgtgtctc ggctgctccc tgtgctgttg
     4141 agcaccgccc aagaggcaga ccccgaggtg cgaagcaatg ccatcttcgg gatgggcgtg
     4201 ctggcagagc atgggggcca ccctgcccag gaacacttcc ccaagctgct ggggctcctt
     4261 tttcccctcc tggcgcggga gcgacatgat cgtgtccgtg acaacatctg tggggcactt
     4321 gcccgcctgt tgatggccag tcccaccagg aaaccagagc cccaggtgct ggctgcccta
     4381 ctgcatgccc tgccactgaa ggaggacttg gaggagtggg tcaccattgg gcgcctcttc
     4441 agcttcctgt accagagcag ccctgaccag gttatagatg tggctcccga gcttctgcgt
     4501 atctgcagcc tcattctggc tgacaacaag atcccaccag acaccaaggc cgcactgttg
     4561 ctgctcctga cgttcctggc caaacagcac accgacagct ttcaagcagc tctgggctca
     4621 ctgcctgttg acaaggctca ggagctccag gctgtactgg gcctctccta gcaattggga
     4681 tcctaatagt ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     4741 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4801 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4861 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4921 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     4981 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5041 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5101 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5161 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5221 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5281 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5341 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     5401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5461 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5521 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5581 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5701 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5761 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5821 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5881 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5941 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6001 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6061 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     6121 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6181 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6241 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6301 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6361 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6421 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6481 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6541 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6601 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6661 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6721 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6781 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6841 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     6901 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     6961 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     7021 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     7081 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     7141 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     7201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7981 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
