LOCUS       sequence-504281-       12364 bp    DNA     circular SYN 27-MAR-2026
DEFINITION  Human expression vector containing EF1a promoter, GFP11 along with a
            leucine zipper, CD19 (no intracellular domain), nuclear BFP, and 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Chen X, Situ Y, Yang Y, Fu ML, Lyu L, Qi LS
  TITLE     Programmable macromolecule delivery via engineered trogocytosis.
  JOURNAL   bioRxiv [Preprint]. 2025 Mar 14:2025.03.12.642522. doi: 
            10.1101/2025.03.12.642522.
  PUBMED    40161588
REFERENCE   2  (bases 1 to 12364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 27, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Mar 14:2025.03.12.642522. doi: 
            10.1101/2025.03.12.642522."
COMMENT     Sequence Label: pSLQ12774-PHR-EF1a-leucine zipper-GFP11-NES-2A...
FEATURES             Location/Qualifiers
     source          1..12364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        271..1449
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          502..1440
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1397..1417
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1481..1490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1610..1657
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1709..1765
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2744..2800
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2801..3394
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3294..3314
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3404..3457
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3470..4165
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             4166..4192
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    4202..4790
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4255..4275)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4673..4684)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4903..5136
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5161..5179)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5162..5179)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5469..5488)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5702..5721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5821..5843
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5881..5899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5967..6071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6072..6140,6141..6932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6290..6309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7103..7691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7592..7611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7845..7862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7932..7952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7937..8266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8117..8252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9400..9465
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9595..9615
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10040..10174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10077..10096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10131..10150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10342..10975
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11022..11147
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11644..11877
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12062..12106
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
       61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttat
      181 cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
      241 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      301 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      361 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      421 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      481 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      541 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      601 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      661 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      721 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      781 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      841 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      901 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
      961 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     1021 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     1081 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     1141 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1201 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1261 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1321 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1381 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1441 gtgtcgtgag gtaccgccta ggaaattctc acgcgtgccc gccaccatgg gatccgagca
     1501 actcgaaaag aagctgcaag cgttggagaa gaagctcgca caacttgaat ggaaaaatca
     1561 ggcactggaa aagaaactgg ctcaaggtgg ctcagggagc ggctcttcac gtgaccacat
     1621 ggtccttcat gagtatgtaa atgctgctgg gattacaggt ggtgggggtt cactgtaccc
     1681 tgagcggctg cggcggatcc tgactagtgc tactaacttc agcctgctga agcaggctgg
     1741 agacgtggag gagaaccctg gacccatgcc acctcctcgc ctcctcttct tcctcctctt
     1801 cctcaccccc atggaagtca ggcccgagga acctctagtg gtgaaggtgg aagagggaga
     1861 taacgctgtg ctgcagtgcc tcaaggggac ctcagatggc cccactcagc agctgacctg
     1921 gtctcgggag tccccgctta aacccttctt aaaactcagc ctggggctgc caggcctggg
     1981 aatccacatg aggcccctgg ccatctggct tttcatcttc aacgtctctc aacagatggg
     2041 gggcttctac ctgtgccagc cggggccccc ctctgagaag gcctggcagc ctggctggac
     2101 agtcaatgtg gagggcagcg gggagctgtt ccggtggaat gtttcggacc taggtggcct
     2161 gggctgtggc ctgaagaaca ggtcctcaga gggccccagc tccccttccg ggaagctcat
     2221 gagccccaag ctgtatgtgt gggccaaaga ccgccctgag atctgggagg gagagcctcc
     2281 gtgtctccca ccgagggaca gcctgaacca gagcctcagc caggacctca ccatggcccc
     2341 tggctccaca ctctggctgt cctgtggggt accccctgac tctgtgtcca ggggccccct
     2401 ctcctggacc catgtgcacc ccaaggggcc taagtcattg ctgagcctag agctgaagga
     2461 cgatcgcccg gccagagata tgtgggtaat ggagacgggt ctgttgttgc cccgggccac
     2521 agctcaagac gctggaaagt attattgtca ccgtggcaac ctgaccatgt cattccacct
     2581 ggagatcact gctcggccag tactatggca ctggctgctg aggactggtg gctggaaggt
     2641 ctcagctgtg actttggctt atctgatctt ctgcctgtgt tcccttgtgg gcattcttca
     2701 tcttcaaaga gccctgggcg gaagcggagg tagtggtgga tccgctacta acttcagcct
     2761 gctgaagcag gctggagacg tggaggagaa ccctggaccc accgagtaca agcccacggt
     2821 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     2881 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     2941 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3001 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3061 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3121 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3181 cggcgtttct ccagaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3241 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3301 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3361 gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg ggagagggca gaggaagtct
     3421 gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg gtcgccacca gcgagctgat
     3481 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     3541 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     3601 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     3661 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     3721 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     3781 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     3841 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     3901 gactctgtat cctgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     3961 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     4021 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     4081 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     4141 tagcaaactg gggcacaagc ttaatcctgc tgctaagaga gtgaaactgg attgagtcga
     4201 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4261 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4321 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4381 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4441 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4501 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4561 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     4621 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4681 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4741 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
     4801 agctcggcct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     4861 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4921 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4981 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5041 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5101 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     5161 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     5221 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     5281 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     5341 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     5401 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     5461 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     5521 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     5581 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     5641 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     5701 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5761 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5821 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5881 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5941 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6001 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6061 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6121 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6181 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6241 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6301 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6361 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6421 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6481 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6541 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6601 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6661 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6721 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6781 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6841 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6901 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6961 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7021 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7081 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7141 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7201 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7261 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7321 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7381 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7441 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7501 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7561 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7621 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7681 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7741 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7801 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7861 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7921 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     8281 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     8341 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     8401 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     8461 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     8521 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     8581 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     8641 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     8701 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     8761 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     8821 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     8881 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     8941 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     9001 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     9061 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     9121 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     9181 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     9241 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     9301 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     9361 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     9421 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     9481 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     9541 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     9601 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     9661 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     9721 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     9781 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     9841 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     9901 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     9961 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10021 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10081 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10141 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10201 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    10261 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    10321 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
    10381 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
    10441 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    10501 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    10561 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
    10621 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
    10681 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
    10741 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
    10801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    10981 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    11041 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    11101 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    11161 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    11221 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    11281 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    11341 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    11401 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    11461 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
    11521 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    11581 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    11641 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    11701 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    11761 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    11821 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    11881 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    11941 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    12001 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    12061 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    12121 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    12181 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    12241 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    12301 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    12361 gtat
//
