LOCUS       sequence-50432-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pAAVsc CB6PI Gpluc7XmiR-122T
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        137..241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             426..804
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(460..479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
       61 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
      121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      241 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      301 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      361 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      421 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      481 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      541 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      601 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      661 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      721 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      781 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      841 ttcccggcaa caattaatag actggatggg aggcggataa agtttgcagg accacttctg
      901 cgctcggccc tttccggctg
//
