LOCUS       sequence-50516-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FES
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaaattccct ctgaatattt tgtttacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgattc
      121 cggaggtgca gaagcccctg catgagcagc tgtggtacca cggggccatc ccgagggcag
      181 aggtggctga gctgctggtg cactctgggg acttcctggt gcgggagagc cagggcaagc
      241 aggagtacgt gctgtcggtg ctgtgggatg gtctgccccg gcacttcatc atccagtcct
      301 tggataacct gtaccgactg gaaggggaag gctttcctag cattcctttg ctcatcgacc
      361 acctactgag cacccagcag cccctcacca agaagagtgg tgttgtcctg cacagggctg
      421 tgcccaagga caagtgggtg ctgaaccatg aggacctggt gttgggtgag cagattggac
      481 gggggaactt tggcgaagtg ttcagcggac gcctgcgagc cgacaacacc ctggtggcgg
      541 tgaagtcttg tcgagagacg ctcccacctg acctcaaggc caagtttcta caggaagcga
      601 ggatcctgaa gcagtacagc caccccaaca tcgtgcgtct cattggtgtc tgcacccaga
      661 agcagcccat ctacatcgtc atggagcttg tgcagggggg cgacttcctg accttcctcc
      721 gcacggaggg ggcccgcctg cgggtgaaga ctctgctgca gatggtgggg gatgcagctg
      781 ctggcatgga gtacctggag agcaagtgct gcatccaccg ggacctggct gctcggaact
      841 gcctggtgac a
//
