LOCUS       sequence-50548-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTGR1
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..78
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             240..251
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct gggtaccgag
       61 aacctgtact tccaatccat gactaagaca tggaccctga agaagcactt tgttggctat
      121 cctactaata gtgactttga gttgaagaca tctgagctcc cacccttaaa aaatggagag
      181 gtcctgcttg aagctttgtt cctcaccgtg gatccctaca tgagagtggc agccaaaaga
      241 ttgaaggaag gtgatacaat gatggggcag caagtggcca aagttgtgga aagtaaaaat
      301 gtagccctac caaaaggaac tattgtactg gcttctccag gctggacaac gcactccatt
      361 tctgatggga aagatctgga aaagctgctg acagagtggc cagacacaat accactgtct
      421 ttggctctgg ggacagttgg catgccaggc ctgactgcct actttggcct acttgaaatc
      481 tgtggtgtga agggtggaga aacagtgatg gttaatgcag cagctggagc tgtgggctca
      541 gtcgtggggc agattgcaaa gctcaagggc tgcaaagttg ttggagcagt agggtctgat
      601 gaaaaggttg cctaccttca aaagcttgga tttgatgtcg tctttaacta caagacggta
      661 gagtctttgg aagaaacctt gaagaaagcg tctcctgatg gttatgattg ttattttgat
      721 aatgtaggtg gagagttttc aaacactgtt atcggccaga tgaa
//
