LOCUS       sequence-50587-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  TITLE     Foxd3-3xFlag RMCE Insertion Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHygroMod-Foxd3-3xFlag
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    338..359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(374..379,380..397,398..404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     417..439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     436..452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     436..452
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        473..491
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(509..542)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(563..634)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      361 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      421 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc
      481 ctcactaaag ggaacaaaag ctggtaccat aacttcgtat aggatacttt atacgaagtt
      541 atccgagatg catgtcgact cacttgtcgt catcgtcctt gtagtccttg tcgtcatcgt
      601 ccttgtagtc cttgtcgtca tcgtccttgt agtcgtcgac ttgagcgggc cacttggctt
      661 gcacggcggc ggccgcagcc gctgcggctg a
//
