LOCUS       sequence-50591-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET-21a(+)/MBP-His
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(62..79)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      262..271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             572..583
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcttatt
       61 agtgatgatg atgatgatga cccagattat tattgttatt gttattatta ttattactag
      121 agctgttcgt ttgagcgtct ttcagtgctt catcaacggt ctggcgacca ctagctgcat
      181 tgatcacggc cgtacggacc gcataccaga aagcagacat ttgcgggatg ttcggcataa
      241 tttcgccttt ctgggcattt tccatggtgg ccgcaatacg cggatctttc accagttctt
      301 cttcatagct tttcagcgcg acagcaccca gcggtttgtc tttgttaacc gcttccaggc
      361 cttcatcggt cagcagataa ttttccagaa attcttttgc cagttcttta ttcgggctag
      421 ctgcgttaat accggccgac agaacgccca cgaacggttt gctcggttga cctttaaacg
      481 tcggcaggac ggtaacgccg tagttaactt ttgaggtatc gatattggac caagcccacg
      541 gaccgttaat cgtcattgcg gtttcgcctt tattgaaggc cgcttcggcg atcgaatagt
      601 ccgtatccgc attcatgtgt ttgtttttaa tcaggtcgac cagaaaggtc agacctgctt
      661 tggcgcccgc gttatccaca ccgacgtctt tgatatcgta tttgccgttt tcgtatttga
      721 acgcgtaacc gccatcagct gcaatcagcg gccacgtgaa atacggttcc tgcaggttaa
      781 acatcagggc tgatttaccc ttcgctttca gttctttgtc cagcgccggg
//
