LOCUS       sequence-50646-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Shen S, Arhin GK, Ullu E, Tschudi C
  TITLE     In vivo epitope tagging of Trypanosoma brucei genes using a one step
            PCR-based strategy.
  JOURNAL   Mol Biochem Parasitol 2001 Mar;113(1):171-3.
  PUBMED    11254965
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biochem
            Parasitol"; date: "2001-03"; volume: "113(1)"; pages: "171-3"
COMMENT     Sequence Label: BLA/V5
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..55)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gggccccctc gagcgtagaa tcgagaccga ggagagggtt agggataggc ttacccataa
       61 ttcgttgact atttgatgaa agggttacta ttctttctcc tctagtaggg ctggtcgaga
      121 aaacaagatt gaaagaaaag gcggaaaggg agagggagaa agaggtgtga cacgacagga
      181 agaagcgaat aaggggaagg aaggcataca cgcaaccaca gacgtgacag ggtgcctcct
      241 acacaaaggg tgagcaaaaa aacaagaaaa acataggtcc caagcgaatt aaaaacattt
      301 gagacagaaa acaaattcac aaaagaaaga aagaaagaac ataaatgaaa acctacacat
      361 ggtgcgacgt tgtcacactt tctaggggta ccgagctcga attggggtgg gcgaagaact
      421 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
      481 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
      541 tcgcttggtc ggtcatttcg aaccccagag tcccgcacgt taattagcac gtgtcagtcc
      601 tgctcctcgg ccacgaagtg cttagccctc ccacacataa ccagagggca gcaattcacg
      661 aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga tcccaggatg
      721 cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt
      781 tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag tgcccagcac
      841 cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag tgaagat
//
