LOCUS       sequence-50700-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Doench JG, Sharp PA
  TITLE     Specificity of microRNA target selection in translational 
            repression.
  JOURNAL   Genes Dev. 2004 Mar 1. 18(5):504-11.
  PUBMED    15014042
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2004 Mar 1. 18(5):504-11."
COMMENT     Sequence Label: pRL-TK let7 G
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    282..403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(293..312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     347..366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        536..640
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cagagtttcc taatctgaat ttgtcaaagt aaaaggtctt catttttcgc aagaagatgc
       61 acctgatgaa atgggaaaat atatcaaatc gttcgttgag cgagttctca aaaatgaaca
      121 ataattctag aaagttttca caaagctaac aggtacctcg agaactatac aacgtctatc
      181 tcaccggaac tatacaacgt ctatctcaac tagtgtacca agttttcaca aagctaacac
      241 cggatcgcgg gccctagagc ggccgcttcg agcagacatg ataagataca ttgatgagtt
      301 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      361 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      421 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      481 ctacaaatgt ggtaaaatcg ataaggatcc aggtggcact tttcggggaa atgtgcgcgg
      541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      781 ggatctcaac agcggtaaga tccttgagag ttttcgcccg agacgttcca tgtgaccttt
      841 aagtcgcagg g
//
