LOCUS       sequence-50750-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Tang X, Germain BS, Lee WL
  TITLE     A novel patch assembly domain in Num1 mediates dynein anchoring at 
            the cortex during spindle positioning.
  JOURNAL   J Cell Biol. 2012 Mar 19;196(6):743-56.
  PUBMED    22431751
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2012-03-19"; volume: "196(6)"; pages: "743-56"
COMMENT     Sequence Label: pBSG01
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata taccatggca aaaatcgaag aaggtaaact
      181 ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt
      241 cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt
      301 cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt
      361 tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga
      421 caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc
      481 gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa
      541 aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct
      601 gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta
      661 tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg
      721 cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga
      781 caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa
      841 cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact
      901 gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctg
//
