LOCUS       sequence-50922-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt1-V5
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    87..111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcattacta accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agatcaacca ctttgtacaa gaaagctggg tcggcgcgcc
      121 cacccttcag aatgcacaga cactcgtgca gtacgcgcgt gtgcgtgcag ttgcggcagc
      181 tgacgtggca gcaccagtgg aaggtgcagt tgcagcgctc ggtgacgcgc tgcgtgcgcg
      241 tgcggtggcc cctgccgcag cagagcagct cgcagccgtc cagcgcgggc gacgagctgt
      301 tacaggcgcg ccctgccgtg cctgctgtgc ccaggcgtcc gctgtacgtg cagaagttgg
      361 gcgatttctc gaagtagacg aggtcgtggg gggagggcgg tttgtgggcc gggtcttccg
      421 gctccaggcg cagcagctcc gcccgcgaag cgcggttgct gccgcggttg ccgtacagga
      481 cgcgcgaggc gccgtcgaag cggtcgcgca gcacatcgcc cacggcgcgc agcgtgggca
      541 gccgcatcca gcacgtgcgc accgtgcatg agccggacat cccgtggcac ttgcactcct
      601 ggcgcatctc ggagaatacg gtcgtacggc ctgcctcgtt gttgtgaagg ttcatgagga
      661 agcgcaggtc ccgccccttc tccccggagt ccacgaactc ccggccgaag aggcggccga
      721 agtcaatgtt gtcgctgcag cccccccagt gccagtcggg gcccccgggg ccgcgccgcc
      781 ggtagtcaca cgtgcaggat tcgatggaac cttctgagca ggagcgcgcc accgaatggg
      841 tgaccccggc ggaagtgata gcgaagataa acgcc
//
