LOCUS       sequence-50941-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt8A-V5
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..53)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(613..624)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctaccggtac gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac
       61 cgcgggccct ctagatcaac cactttgtac aagaaagctg ggtcggcgcg cccacccttc
      121 agaatgcagg cactgccctt acccagggac tgggcactgc ctggggagcg tgcgcagtaa
      181 tacttgctca ccacatgcct acactggtca cacttgaccg tacagcacca ctggaatttg
      241 cagttacagc tgcttatgac ctcagttttc ctctcttcca cctgcagccc acactcagtg
      301 cacaggcgcc cacagctacg tcgctcccac ctggatgtgt tgtggctgtt ctgtaggcac
      361 tcacgaccct ctgtgccata gatgcccagg ctggaattgc aggtacagta atctggtgat
      421 tcctctaaaa agatcagttc cgcctctgcg ctaggaagga aggcctcagc gggcacccag
      481 tggccctcgg cgctgttccc agctctcagc tgccgcttat ccatttcaat tttcagcgcc
      541 tggtcatact tggcctttag gtagtctccc atctcccgga actcagccag ctgcagccag
      601 catgtctgta tgctgcagct cccagagatg ccatgacatt tgcatgtcct tttcatggtg
      661 gctctcactg ccagtctgcc ggccctgttg ttgtgaagat tcatcagggc tctggcatcc
      721 ttccccttct ccaaactgtc cacaaagagt ttggagatcc tttccccaaa ttccacattg
      781 tcgctgcagc ctccccagat ccagccatgg cctcctgttt ttccattgtt tgacccatca
      841 cagccacagt tttcgaagtc acccatgcta cagttcttgg tgatgatgta
//
