LOCUS       sequence-50943-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt11-V5
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..53)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actaccggta gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac
       61 cgcgggccct ctagatcaac cactttgtac aagaaagctg ggtcggcgcg cccacccttc
      121 agaatgcact tgcagacata gcgctccacg gtacgctcac acctgcggca ggtgacgtag
      181 cagcaccagt ggtacttaca gtggcaccgc tcgaccacgc ggtctgtgta ggggttgtag
      241 ccacgcccgc agcacataag gtcgcagctg tcgcttccgt tggatgtctt gttgcactgc
      301 ctgtcttgtg tcccgtggga gcccaccttc tcattcttca tgcagaagtc aggtgagctc
      361 tgcagataga cgagttccga gtccttcaca ggccggatat ccaggtcctt gggcaccagg
      421 tgcttgcggg tgcccatggg tcggtgcact accttggtgg ccgacaggta tcgggtcttg
      481 aggtcagcag ccacatcctg cagctcctgc agccccttcc agcaggtgcg gatggagcag
      541 gagccagaca ccccatggca cttacacttc atttccagag aggcgcgcag agcctgtctc
      601 cccacttcac tgttgtgtag acgcatcagt ttattggctt gggatcctgt ttttttcacc
      661 ttcataggag catcggaaaa cttggccccc atgaggagcc cgtagctgag gttgtccgca
      721 catcctcccc agcggttccc gggcccgggt ggctcacctg ggacggggcc gcaggagcag
      781 ccgggcaggt cgccggaggt gcaggcccgg gcgatggcgt ggctgatggc ggcggccgac
      841 agcgcataca cgaaggccga ctcccgggtc
//
