LOCUS       sequence-50999-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of GFP under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-GFP
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    28..616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(81..101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(499..510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(670..689)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     724..743
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
       61 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      121 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      181 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
      241 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
      301 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
      361 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
      421 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
      481 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
      541 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
      601 tgggccgcct ccccgcatcg ataccgtcga cccgggcggc cgcttcgagc agacatgata
      661 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      721 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa ta
//
