LOCUS       sequence-51031-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P3nmt1-3HA
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(43..230)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     151..175
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(263..352)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 ggtcgacgga tctatattac cctgttatcc ctagcggatc tgccggtaga ggtgtggtca
       61 ataagagcga cctcatacta tacctgagaa agcaacctga cctacaggaa agagttactc
      121 aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta tacacttatt
      181 ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc ttatttagaa
      241 gtggcgcgcc tcagcactga gcagcgtaat ctggaacgtc atatggatag gatcctgcat
      301 agtccgggac gtcataggga tagcccgcat agtcaggaac atcgtatggg taaaagatgt
      361 taattaaaga catgatttaa caaagcgact ataagtcaga aagtgagaat gagattgaaa
      421 taattaattc aacttattca attgatgata tgccaggatt cctcttcctt tatatatctt
      481 caatgtttcg attaacaggt ccattatccg gtttttagtt tcgctgcaca ttgccgaatg
      541 acaatcggat tcctttaagc tttgcttttg ccctgttttc tctccccagt aatcgttggg
      601 tgactgaacc atccagtagc cgagagttac gtttttcctg atttaataaa tatccatatc
      661 tatttgtctc gtggtgtttt acagcaacta accgaaatta ctattttgca tcatccaacc
      721 ataaagagac aacaataatt ctagaaaaca tataacaagt gtttttttct atttacgtct
      781 catacgcaaa gtccacttta ccatgttttg gaaacttgta cccaa
//
