LOCUS       sequence-51037-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Lautz K, Damm A, Menning M, Wenger J, Adam AC, Zigrino P, Kremmer E,
            Kufer TA
  TITLE     NLRP10 enhances Shigella-induced pro-inflammatory responses.
  JOURNAL   Cell Microbiol. 2012 Jun 4. doi: 10.1111/j.1462-5822.2012.01822.x.
  PUBMED    22672233
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Microbiol. 2012 Jun 4. doi: 10.1111/j.1462-5822.2012.01822.x."
COMMENT     Sequence Label: FLAG-NLRP10
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact aaagggaaca
       61 aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat gacgacgata
      121 agagcccggg cggatccatg gccatggcca aggccagaaa gccccgggag gcattgctct
      181 gggccttgag tgaccttgag gagaacgatt tcaagaagtt aaagttctac ttacgggata
      241 tgaccctgtc tgagggccag cccccactgg ccagagggga gttggagggc ctgattccgg
      301 tggacctggc agaattactg atttcaaagt atggagaaaa ggaggctgtg aaagttgtcc
      361 tcaagggctt gaaggtcatg aacctgttgg aacttgtgga ccagctcagc catatttgtc
      421 tgcatgatta cagagaagta taccgagagc atgtgcgctg cctagaggaa tggcaggaag
      481 caggagtcaa tggcagatac aaccaggtgc tcctggtggc caagcccagc tcagagagcc
      541 cagaatcact tgcctgcccc ttcccggagc aggagctgga gtctgtcacg gtggaggctc
      601 tatttgattc aggggaaaag ccctcactgg ccccatcctt agttgtgcta caggggtcgg
      661 ctggcactgg aaagacaact ctcgccagaa aaatggtgtt ggactgggcc accggtactc
      721 tgtacccagg ccggtttgat tatgtctttt atgtaagctg caaagaagtg gtcctgctgc
      781 tggagagcaa actggagcag c
//
