LOCUS       sequence-51083-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Downward Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBABEpuro-H-RAS_V12
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(34..66,67..69,70..603)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(763..783)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtcagcttgg ggggccaggg ccggggccga gcgatgacgg aatataagct ggtggtggtg
       61 ggcgccgtcg gtgtgggcaa gagtgcgctg accatccagc tgatccagaa ccattttgtg
      121 gacgaatacg accccactat agaggattcc taccggaagc aggtggtcat tgatggggag
      181 acgtgcctgt tggacatcct ggataccgcc ggccaggagg agtacagcgc catgcgggac
      241 cagtacatgc gcaccgggga gggcttcctg tgtgtgtttg ccatcaacaa caccaagtct
      301 tttgaggaca tccaccagta cagggagcag atcaaacggg tgaaggactc ggatgacgtg
      361 cccatggtgc tggtggggaa caagtgtgac ctggctgcac gcactgtgga atctcggcag
      421 gctcaggacc tcgcccgaag ctacggcatc ccctacatcg agacctcggc caagacccgg
      481 cagggagtgg aggatgcctt ctacacgttg gtgcgtgaga tccggcagca caagctgcgg
      541 aagctgaacc ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc
      601 tgacgcagca caagctcagg acatggaggt gccggatgca ggaaggaggt gcagacggaa
      661 ggaggaggaa ggaaggacgg aagcaaggaa ggaaggaagg gctgctggag cccagtcacc
      721 ccccaagctg taggaattcg ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag
      781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      901 tgcatctcaa ttagtcagcc aa
//
