LOCUS       sequence-51128-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-GST-kanMX6
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..688
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     646..668
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(785..809)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taatatgtcc cctatactag gttattggaa
       61 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
      121 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
      181 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
      241 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
      301 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
      361 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
      421 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
      481 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
      541 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
      601 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
      661 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgttgag gcgcgccact
      721 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
      781 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
      841 agtaactctt tcctgtaggt caggttgctt t
//
