LOCUS       sequence-51135-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-3HA-kanMX6
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..120
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      153..340
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(208..232)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        415..758
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(826..845)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgacggatcc ccgggttaat taacatcttt tacccatacg atgttcctga ctatgcgggc
       61 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
      121 gctcagtgct gaggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
      181 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
      241 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
      301 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca
      361 gggtaatata gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
      421 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
      481 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
      541 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
      601 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
      661 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
      721 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg
      781 tttcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      841 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatggg
//
