LOCUS       sequence-51169-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Najdi R, Proffitt K, Sprowl S, Kaur S, Yu J, Covey TM, Virshup DM, 
            Waterman ML
  TITLE     A uniform human Wnt expression library reveals a shared secretory 
            pathway and unique signaling activities.
  JOURNAL   Differentiation. 2012 Sep;84(2):203-13. doi: 
            10.1016/j.diff.2012.06.004. Epub 2012 Jul 9.
  PUBMED    22784633
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.diff.2012.06.004"; journalName: "Differentiation"; date: 
            "2012-09"; volume: "84"; issue: "2"; pages: "203-13"
COMMENT     Sequence Label: pcDNA-Wnt2-V5
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..57)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttactaaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc
       61 gaaccgcggg ccctctagat caaccacttt gtacaagaaa gctgggtcgg cgcgcccacc
      121 cttcagaatg catgtagcgg ttgtccagtc agcgttcttg ggggccttgc atgtgtgcac
      181 atccagagct tccaggcagt cctgacagcg cacggcgcag caccagtgga acttacaccc
      241 acacttggtc atccgggtga catgggaggt gtcgtagcct ctcccacagc acatgacttc
      301 acagctgtcc atgccccggg aagtcaggtt gcacacacgg cctgctgtac ccagggagcc
      361 tgcctctcgg tccctgatac agtagtctgg agaattttca aaatacacga ggtcattttt
      421 cgttggcttc ttaaacctct cgttagccac agtgaaacct gtgccatcct ggttcatgac
      481 cacctggatg gccccattgt acttcctcca gagataatcg cccgttttcc tgaagtcggc
      541 catggccagc cagcatgtcc tgagagtaca tgagccgctc accccgtggc acttgcactc
      601 ttgtttcaag aaccgcttta cagccttcct gccagctctg ttgttgtgaa gattcatcag
      661 ggctctggca tcctttcctt tcctttcctt tgcatccaca aatgcgcggg caaatttgat
      721 cccatagtca atgttatcac tgcagccacc ccaatcaaaa atgcctttgc tgtccttggc
      781 gcttcccatc ttctttggat cacaggaaca gga
//
