LOCUS       sequence-51220-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TWF2
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             565..582
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(635..653)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      649..696
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(820..839)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgggcatcc acgccacgga
      121 agagctgaag gaattctttg ccaaggcacg ggctggctct gtgcggctca tcaaggttgt
      181 gattgaggac gagcagctcg tgctgggtgc ctcgcaggag ccagtaggcc gctgggatca
      241 ggactatgac agggccgtgc tgccactgct ggacgcccag cagccctgct acctgctcta
      301 ccgcctcgac tcacagaatg ctcagggctt cgaatggctc ttcctcgcct ggtcgcctga
      361 taactccccc gtgcggctga agatgctgta cgcggccacg cgggccacag tgaaaaagga
      421 gtttggaggt ggccacatca aggatgagct cttcgggact gtgaaggatg acctctcttt
      481 tgctgggtac cagaaacact gacagtaaag gtggatacgg atccgaattc gagctccgtc
      541 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      601 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      661 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      721 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      781 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      841 cctttctcgc cacgttcgcc gggctttccc cgtcaagctc taaatcgggg gctcccttta
      901 ggt
//
