LOCUS       sequence-51472-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Mansfeld J, Collin P, Collins MO, Choudhary JS, Pines J
  TITLE     APC15 drives the turnover of MCC-CDC20 to make the spindle assembly 
            checkpoint responsive to kinetochore attachment.
  JOURNAL   Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347.
  PUBMED    21926987
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-09-18- 18"; volume: "13"; issue: "10"; 
            pages: "1234-43"
COMMENT     Sequence Label: pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(550..574)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    584..602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    605..623
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(625..829)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(654..674)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgagtact tggtcatcgt catccttgta atcgatgtca tgatctttat aatcaccgtc
       61 atggtctttg tagtcgatca tccactggtc ctgatcctgt tcgttgcctt ccatgtccac
      121 ctcattgacc tctccatcat ccggtgactc attgtagtca ttcatctcgt ccatgtcctg
      181 catatcctca tcatcctctg agtcctcttc actatcctca tcatcttcat catcctcttc
      241 ttcctcgtca tcatagtgct ctgaggctgg cttgccaata ggaaccaggt tgttgtcttt
      301 ctccgcgatg ctttggagcc aggcctgatg ctgctgttcc tgctgctgca gctctgtctc
      361 ttccacacag ggtcgatcca gattaaacca cagagtctca gtcacacgag ggaagagtga
      421 ggggaacaaa gtggacatgg tggatccgag ctcggtacca agcttaagtt taaacgctag
      481 agtccggagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg
      541 tctccaggcg atctgacggt tcactaaacg agctcgtcga cgatctctat cactgatagg
      601 gagatctcta tcactgatag ggagagctct gcttatatag acctcccacc gtacacgcct
      661 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      721 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      781 gtcaaaccgc tatccacgcc ccattgatgt actgccaaaa ccgcatcacc catgggtaat
      841 agcgatgact aatacgtaga tgtactgcca agtaggaaaa gtcccataag gtcatg
//
