LOCUS       sequence-51495-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Bokoch Lab GTPases
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRK5-myc-RhoA-wt
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..88
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             125..706
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     regulatory      726..735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    739..820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(776..795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggtgtccact cccaggtcca actgcacctc ggttctatcg attgaattgg ccaccatgga
       61 gcagaagctg atctccgagg aggacctggg atccatggag tatgatgttc ctgattatgc
      121 tcatatggct gccatccgga agaaactggt gattgttggt gatggagcct gtggaaagac
      181 atgcttgctc atagtcttca gcaaggacca gttcccagag gtgtatgtgc ccacagtgtt
      241 tgagaactat gtggcagata tcgaggtgga tggaaagcag gtagagttgg ctttgtggga
      301 cacagctggg caggaagatt atgatcgcct gaggcccctc tcctacccag ataccgatgt
      361 tatactgatg tgtttttcca tcgacagccc tgatagttta gaaaacatcc cagaaaagtg
      421 gaccccagaa gtcaagcatt tctgtcccaa cgtgcccatc atcctggttg ggaataagaa
      481 ggatcttcgg aatgatgagc acacaaggcg ggagctagcc aagatgaagc aggagccggt
      541 gaaacctgaa gaaggcagag atatggcaaa caggattggc gcttttgggt acatggagtg
      601 ttcagcaaag accaaagatg gagtgagaga ggtttttgaa atggctacga gagctgctct
      661 gcaagctaga cgtgggaaga aaaaatctgg ttgccttgtc ttgtgagaat tcctgcagaa
      721 gcttggccgc catggcccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      781 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tgt
//
