LOCUS       sequence-51602-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-GST-kanMX6
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(40..383)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      complement(458..645)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     566..590
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(707..729)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ttatcgcggc ctcgaaacgt gagtcttttc cttacccatg gttgtttatg ttcggatgtg
       61 atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa gaacctcagt
      121 ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca attcaacgcg
      181 tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta atttttgctt
      241 cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc
      301 taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg
      361 agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagctaa
      421 acagatctat attaccctgt tatccctagc ggatctgccg gtagaggtgt ggtcaataag
      481 agcgacctca tactatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
      541 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
      601 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtggc
      661 gcgcctcaac gcggaaccag atccgatttt ggaggatggt cgccaccacc aaacgtggct
      721 tgccagccct gcaaaggcca tgctatatac ttgctggatt tcaagtactt atcaatttgt
      781 gggatagctt caatacgttt tttaaaacaa actaattttg ggaacgcatc caggcacatt
      841 gggtccatgt ataaaacaac atcaagagcg tcatacaaca tgaagtcagg atgggttaca
      901 tgatcaccat ttaaatatgt tttatgacat aaacgatctt cgaacatttt cagcatttca
      961 ggtagcttgc taagaaaatc aactttgaga gtttcaa
//
