LOCUS       sequence-51607-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3330 (=HL 770 + pHL 1009)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     78..96
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(216..244)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     839..859
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
       61 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagtccc
      121 tatcagtgat agagattgac atccctatca gtgatagaga tactgtcgac taaggaggaa
      181 aaaaaatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      241 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      301 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      361 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      421 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      481 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      541 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      601 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      661 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      721 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      781 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      841 tccttcttga gtttgtaaca gctgctggga ttacacatgg gcatgg
//
