LOCUS       sequence-51637-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Downward Lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEXV3-Myc-H-RAS_N17
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          85..181
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             235..261
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             270..299
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     947..966
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catcagagta gtgaggagct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga
       61 actgaaaaac cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc
      121 ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta
      181 ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg aattcggagc gatgtaccca
      241 tacgatgttc cagattacgc tccaccatgg aacagaaact catttccgaa gaggatctga
      301 agcttgttgt tgttggcgcc ggcggtgtgg gcaagaatgc gctgaccatc cagctgatcc
      361 agaaccattt tgtggacgaa tacgacccca ctatagagga ttcctaccgg aagcaggtgg
      421 tcattgatgg ggagacgtgc ctgttggaca tcctggatac cgccggccag gaggagtaca
      481 gcgccatgcg ggaccagtac atgcgcaccg gggagggctt cctgtgtgtg tttgccatca
      541 acaacaccaa gtcttttgag gacatccacc agtacaggga gcagatcaaa cgggtgaagg
      601 actcggatga cgtgcccatg gtgctggtgg ggaacaagtg tgacctggct gcacgcactg
      661 tggaatctcg gcaggctcag gacctcgccc gaagctacgg catcccctac atcgagacct
      721 cggccaagac ccggcaggga gtggaggatg ccttctacac gttggtgcgt gagatccggc
      781 agcacaagct gcggaagctg aaccctcctg atgagagtgg ccccggctgc atgagctgca
      841 agtgtgtgct ctcctgacgc gaattccgat ccagacatga taagatacat tgatgagttt
      901 gggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      961 tattgcttta tttg
//
