LOCUS       sequence-51684-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: REM1
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(640..660)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(685..702)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             713..735
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    750..774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccgt atccaccttt actgtcacgc
      121 actgtcccgg cggcgcaagc gcagttggcg caccacgccc tcgaagagct cggccacatt
      181 gtgctgcagc gtggcggatg tctcgatgaa tttacagtcg aacaccacag cgcaggcgcg
      241 gccctcttcc acagagactt ctcggcagcg ggccaagtct gccttgttgc ccacgagtat
      301 gatgggcaca tggtctgcct gatgtgtgcg ccgcagctgg atgcggagct cagaggcact
      361 ctcaaagctg cctcggtctg cgatggagta tacgatgaca taggcactgc ccccctgcag
      421 gcatgactcc tggctccagc ttttatccag tttctcggcc tcccaggtgt ccacgaccac
      481 cagtgtggtg tcttctccat ccaccgtgag ggtcctctca tatacatctt ctcccagctg
      541 ttcatggagg tccctctctt gcttccctgc aaagaggctg gccaagctgg tcttccccac
      601 tccaggatct ccaagtagca ccacacggta gagagccatg gattggaagt acaggttctc
      661 ggcacccaga tctacaccag aagaatgatg atgatgatgg tgcatatgta tatctccttc
      721 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      781 gtgagtcgta t
//
