LOCUS       sequence-51685-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: REM1
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             686..703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(756..774)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      770..817
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtgccgag aacctgtact tccaatccat ggctctctac cgtgtggtgc
      121 tacttggaga tcctggagtg gggaagacca gcttggccag cctctttgca gggaagcaag
      181 agagggacct ccatgaacag ctgggagaag atgtatatga gaggaccctc acggtggatg
      241 gagaagacac cacactggtg gtcgtggaca cctgggaggc cgagaaactg gataaaagct
      301 ggagccagga gtcatgcctg caggggggca gtgcctatgt catcgtatac tccatcgcag
      361 accgaggcag ctttgagagt gcctctgagc tccgcatcca gctgcggcgc acacatcagg
      421 cagaccatgt gcccatcata ctcgtgggca acaaggcaga cttggcccgc tgccgagaag
      481 tctctgtgga agagggccgc gcctgcgctg tggtgttcga ctgtaaattc atcgagacat
      541 ccgccacgct gcagcacaat gtggccgagc tcttcgaggg cgtggtgcgc caactgcgct
      601 tgcgccgccg ggacagtgcg tgacagtaaa ggtggatacg gatccgaatt cgagctccgt
      661 cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      721 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      781 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      841 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtgg
//
