LOCUS       sequence-51688-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DLG3
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(358..378)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(403..420)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             431..453
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    468..492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(492..511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(493..511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(595..614)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg
       61 tatccacctt tactgtcagc tgcctggctt ggccaccttc aaatacacca tatcagatgt
      121 gttcttcagt gaggccacag cttcctcgtg cctcacatcc tgcagattgg tgttgttcac
      181 cgccagcagc cggtccccaa tctgtaggcg tccatccttc tgagcagcac ccccctcaat
      241 gatcttggtg atgtagatgc tgttgtctcc tgggatgtgc tggttgccaa tacccccagc
      301 aatgctgaaa cccaggcctt tgggcccttt gagcaggttg acctccatga tggtcatgga
      361 ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg
      421 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      481 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
      541 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
      601 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
      661 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
      721 atgcaccatt ccttgcggcg gcgggtgctc aacggcctca acctactact gggctgcttc
      781 ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gaaca
//
