LOCUS       sequence-51754-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAP3K10
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             378..395
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(448..466)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      462..509
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(633..652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     843..864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgggcacg
      121 acccccgcgg ggcccgtctg gaccgcggtg ttcgactacg aggcggcggg cgacgaggag
      181 ctgaccctgc ggaggggcga tcgcgtccag gtgctttccc aagactgtgc ggtgtccggc
      241 gacgagggct ggtggaccgg gcagctcccc agcggccgcg tgggcgtctt ccccagcaac
      301 tacgtggccc cctgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc
      361 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      421 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct a
//
