LOCUS       sequence-51809-e        8562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Hafner M, Landthaler M, Burger L, Khorshid M, Hausser J, Berninger 
            P, Rothballer A, Ascano M Jr, Jungkamp AC, Munschauer M, Ulrich A, 
            Wardle GS, Dewell S, Zavolan M, Tuschl T
  TITLE     Transcriptome-wide identification of RNA-binding protein and 
            microRNA target sites by PAR-CLIP.
  JOURNAL   Cell. 2010 Apr 2;141(1):129-41.
  PUBMED    20371350
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2010-04-2"; volume: "141(1)"; pages: "129-41"
COMMENT     Sequence Label: pFRT/FLAG/HA-DEST PUM2
FEATURES             Location/Qualifiers
     source          1..8562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..42
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(86..105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        277..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        657..860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     811..831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    862..880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    883..901
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     911..935
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1035..1058
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1059..1085
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1101..1125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4358..4382)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4435..4476
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4514..4531)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4520..4744
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4877..4896)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5028..5075
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5083..6105
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5083..6105
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6235..6356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6272..6291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6326..6345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6405..6421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6405..6421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6418..6440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6429..6445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6453..6459,6460..6477,6478..6483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6498..6519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6636..6653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6807..7395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6887..6906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7566..8357,8358..8426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8189..8208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8427..8531)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggaa ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      901 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      961 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
     1021 agcttggtac catggactac aaggacgacg atgacaagta cccttatgac gtgcccgatt
     1081 acgctttgaa ccagatatca acaagtttgt acaaaaaagc aggctccacc atgggaacca
     1141 tgaatcatga ttttcaagct cttgcattag aatctcgggg aatgggagag cttttgccta
     1201 ccaaaaagtt ttgggaacct gatgattcaa caaaagatgg acaaaaaggc atatttcttg
     1261 gggatgatga atggagagag actgcatggg gagcttctca ccattcaatg tcccagccta
     1321 ttatggtaca gagaagatct ggacagggtt ttcatggaaa cagtgaagta aatgcaatac
     1381 tgtctccgcg atcagaaagt ggaggccttg gtgtgagcat ggtagaatat gtattaagtt
     1441 cttctcctgc tgataaattg gattctcgat ttaggaaggg aaattttggc actagagatg
     1501 ctgaaacaga tggacctgag aaaggagatc aaaaaggcaa ggcttctcca tttgaggagg
     1561 accaaaacag agaccttaaa caaggagatg atgatgattc taaaataaat ggcagaggtt
     1621 tgccaaatgg aatggatgcc gattgcaaag attttaatcg tactcctgga agtcgtcaag
     1681 cctctccaac tgaagtagtt gagcgcttgg gccccaatac taatccctca gaaggactgg
     1741 ggcctcttcc taatcctaca gctaataaac cacttgttga agaattttca aatcctgaaa
     1801 ctcagaatct ggatgccatg gaacaagttg gtctggaatc cttacagttt gactatcctg
     1861 gtaatcaggt accaatggac tcttcaggag ctactgtagg cctttttgac tacaattccc
     1921 agcagcagct ctttcagagg actaatgcac taacagttca acagttaact gcagctcaac
     1981 agcagcaata tgcattagca gcagctcagc agccacatat agctggtgta ttctcagcag
     2041 gccttgctcc agctgcattt gtgccaaatc catacattat tagtgctgct cctccaggga
     2101 ccgatccgta tactgcagca ggattggctg cagcagctac attagcaggt ccagcagtgg
     2161 ttccacctca gtattacggc gttccatggg gggtgtatcc agccaactta tttcagcagc
     2221 aagctgcagc tgcggcaaat aacacagcca gtcagcaagc agcatcacaa gctcagcctg
     2281 gacagcaaca ggttctccgt gctggagcag gtcagcgtcc tcttactccc aatcagggtc
     2341 agcaagggca gcaagcagaa tcacttgcgg cagctgcagc agcaaatcca acattggctt
     2401 ttggtcaggg tcttgctact ggcatgccag gctatcaagt actagctcca actgcctatt
     2461 atgatcagac tggtgcctta gtggttggcc ctggagcaag gactggcctt ggagctccag
     2521 ttcggttaat ggctccaaca cctgttttaa ttagttcagc agcagcacaa gctgcagcag
     2581 cagcagcagc tggaggaact gcaagtagcc ttacaggcag cacaaatggt ctgtttcggc
     2641 caattggcac tcagccacca cagcagcagc aacagcagcc aagcactaat ctgcaatcta
     2701 attcatttta tggaagcagt tctttgacta atagctccca gagtagttct ttattttctc
     2761 atggacctgg tcaacctgga agtacatctc ttggctttgg aagtggtaac tctttgggtg
     2821 ctgctatagg ctcagccctc agtggatttg gttcatcagt tggcagttct gcaagtagta
     2881 gtgccacaag gagagagtct ctatctacta gctctgactt gtacaaaaga tctagtagca
     2941 gcctagcacc catagggcaa ccattttaca atagtctggg attttcctcc tctccaagtc
     3001 caataggcat gcctctgcca agccaaactc caggacattc acttacgcca ccgccatcac
     3061 tttcatcaca tggatcctca tccagtttgc atttaggagg actgacaaat ggtagtggtc
     3121 gatatatctc tgcagcacct ggagcagaag caaaatatcg aagtgcttca agcacttcca
     3181 gtctatttag ctccagcagc cagctctttc ctccttcccg gcttcggtat aataggtctg
     3241 atattatgcc ttctggccgc agtagattat tggaagattt cagaaacaac cgcttcccaa
     3301 accttcagct tagagacttg attggacata tagttgagtt ttctcaagac cagcatggtt
     3361 ctagattcat acagcaaaaa ctagagagag ctactccagc tgagcgacag atggtattta
     3421 atgaaattct gcaagcagcc tatcaattaa tgactgatgt ttttggcaac tatgttatac
     3481 agaagttttt tgagtttggg agtctggatc aaaaattagc cctggctact cgtattcgtg
     3541 gtcatgttct acccttagcc ttgcagatgt atggctgccg cgttattcag aaagcattag
     3601 aatctatttc ttctgaccag cagagtgaaa tggtaaagga gctggatggt catgtgctca
     3661 aatgtgtgaa agatcagaat ggaaaccatg ttgtacaaaa atgtatcgaa tgtgttcagc
     3721 cacagtcact acagttcatc attgatgctt tcaagggaca agtatttgtg ctttcaactc
     3781 atccttatgg ctgcagagta attcagcgca tcctagagca ttgcactgca gaacagacct
     3841 tacctatctt agaagaactc caccaacata cagagcagtt ggtacaggat cagtatggca
     3901 attatgttat tcagcatgta ctggaacacg gtcgacctga agacaagagc aaaattgttt
     3961 ccgaaatcag gggaaaggtt ttagccctga gtcaacacaa atttgccagc aatgtagtag
     4021 aaaagtgtgt tactcatgcc tcccgtgctg agagagcttt actgattgac gaggtttgct
     4081 gccagaatga tggtcctcac agtgccttat acaccatgat gaaggaccag tatgccaatt
     4141 acgtggttca aaagatgatt gatatggctg aacctgctca gagaaagata atcatgcaca
     4201 agattcgacc tcacattact actttgcgca aatacacata cgggaagcat atactggcca
     4261 agttggaaaa gtattatttg aagaatagcc cggacctagg acctattgga ggaccaccaa
     4321 atggaatgct gtaagcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtg
     4381 gttgatatcc agcacagtgg cggccgctca agtctagagg gcccgcggtt cgaaggtaag
     4441 cctatcccta accctctcct cggtctcgat tctacgctcg agtctagagg gcccgtttaa
     4501 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4561 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4621 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4681 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4741 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4801 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4861 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4921 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4981 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc
     5041 cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc
     5101 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     5161 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     5221 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     5281 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     5341 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     5401 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     5461 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     5521 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     5581 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     5641 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     5701 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     5761 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     5821 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     5881 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     5941 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     6001 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     6061 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat
     6121 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6181 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6241 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6301 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6361 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8521 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
