LOCUS       sequence-51814-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPSB1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(745..765)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(790..807)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             818..840
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    855..879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(879..898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(880..898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat ccgtatccac ctttactgtc actcgggatc
      121 gagtccgttc aagtagcgca ttcggatctc acagtggccc cagacggcac tcactacagg
      181 atacagtttt ttgcccttga gtccccgaaa agccactccc atgtactgtc catccacaat
      241 gaagctcaga gtcccgtcgt ccatgtccag ggctaccagg aaggagtcag ggacaatgaa
      301 tgtctcatct ggttccagaa aggctgggta tgttttgctt ggctggttct tgccatcgtg
      361 gtagagccgg ttgcgcccca agtcccagcc ccaggactcg tggttattcc ccacgagggt
      421 tgtgtacccg acagagtgca ggggggcgtc tgccgtcgcc acccccacca cggcgtgtgt
      481 gccccgctgt ctcatggccc acgtgatctg ccacacgtgc agcccacggg tatacccgac
      541 tttgcccctg atagcgtccg tgctctgggc caccggatgc cggtgaaaga tgagcttgtc
      601 gtcctccttc acaaagacat tgagcgatcg gtcgttgttg ttccatgaat gcagcagctg
      661 gacatcatag gacacagggg gcatgtccag tagcagatcc agccgggtgg gcttgcagta
      721 atccagaccc tggagctcct gcatggattg gaagtacagg ttctcggtac ccagatctac
      781 accagaagaa tgatgatgat gatggtgcat atgtatatct ccttcttaaa gttaaacaaa
      841 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtatta
//
