LOCUS       sequence-51864-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BRD1
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             573..590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(643..661)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      657..704
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(828..847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga gcaggtcgcc
      121 atggagctgc ggctgacccc gctgacggtg ctgctgcgct cagtgctgga ccagctgcaa
      181 gacaaggacc ccgccaggat atttgcgcag cccgtgagtc tgaaggaggt accagattat
      241 ttggatcaca ttaaacatcc catggacttt gccacaatga ggaaacggtt agaagctcaa
      301 gggtataaaa acctccatga gtttgaggag gattttgatc tcattataga taactgcatg
      361 aagtacaatg ccagggacac cgtgttctat agagccgcgg tgaggctgcg cgatcaggga
      421 ggtgttgttc tgaggcaggc ccggcgcgag gtggacagca tcggcttgga agaggcctcg
      481 gggatgcacc tgcctgagcg gcctgcttga caataaaggt ggatacggat ccgaattcga
      541 gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
      601 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      661 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      721 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      781 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      841 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggc
//
