LOCUS       sequence-51874-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: JMJD6
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(654..671)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgaaccaca agagcaagaa gcgcatccgc
      121 gaggccaagc ggagtgcgcg gccggagctc aaggactcgc tggattggac ccggcacaac
      181 tactacgaga gcttctcgct gagcccggcg gccgtggcgg ataacgtgga aagggcagat
      241 gctttacagc tgtctgtgga agaatttgtg gagcggtatg aaagacctta caagcccgtg
      301 gttttgttga atgcgcaaga gggctggtct gcgcaggaga aatggactct ggagcgccta
      361 aaaaggaaat atcggaacca gaagttcaag tgtggtgagg ataacgatgg ctactcagtg
      421 aagatgaaga tgaaatacta catcgagtac atggagagca ctcgagatga tagtcccctt
      481 tacatctttg acagcagcta tggtgaacac cctaaaagaa ggaaactttt ggaagactac
      541 aaggtgccaa agtttttcac tgatgacctt ttccagtatg ctggggagaa gcgcaggccc
      601 ccttacaggt ggtttgtgat ggggccacca cgctccggaa ctgggattca catcgaccct
      661 ctgggaacca gtgcctggaa tgccttagtt cagggccaca agcgctggtg cctgtttcct
      721 accagcactc ccagggaact catcaaagtg acccgagacg aaggagggaa ccagcaagac
      781 gaagctatta cctggtttaa tgttatttat ccccggacac agcttccaac ctggccacct
      841 gaattcaaac ccctggaaat cttacaaaa
//
