LOCUS       sequence-52039-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E
  TITLE     A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive 
            Bacterial Immunity.
  JOURNAL   Science. 2012 Aug 17;337(6096):816-21. doi: 10.1126/science.1225829.
            Epub 2012 Jun 28.
  PUBMED    22745249
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1225829"; journalName: "Science"; date: 
            "2012-08-17- 17"; volume: "337"; issue: "6096"; pages: "816-21"
COMMENT     Sequence Label: pMJ823
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..354
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             376..393
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             406..426
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttgtttact ttagaaggag atataccatg agcgataaaa ttattcacct gactgacgac
       61 agttttgaca cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag
      121 tggtgcggtc cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag
      181 ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat
      241 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
      301 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccggatct
      361 ggcagtggtt ctggtcatca ccatcaccat cactccgcgg gtagcgagaa tctttatttt
      421 cagggcgcca tggaaaaacc gtacacgatt ggactagaca taggaacaaa ctcggttgga
      481 tgggcagtgt taacagatca atatgattta gtgaagagga aaatgaaaat tgctggagac
      541 tctgagaaaa aacagataaa gaaaaatttc tggggagtta gactatttga tgagggtcaa
      601 acggcagctg atagaagaat gactagaaca gctagaagga gaatagagag aagacgcaat
      661 cgtatttcgt atttacaagg gatatttgcg gaagaaatgt ctaaggtgga tgcaaatttc
      721 ttttgccgat taagtgatag cttttatgtt gataacgaaa aaagaaatag tcgtcatcct
      781 ttttttgcga ctatagagga agaagtagaa taccacaaaa actaccc
//
