LOCUS       sequence-52191-e        9470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1510_2187
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5520..5537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5574..6931
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5590..6931)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7023..7040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7194..7782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7274..7293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7953..8744,8745..8813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8576..8595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8814..8918)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8945..9400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9032..9051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9242..9263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccaacttcaa aattcgcgac
     3481 cagctggtgg tcggacagct gatccccgac tgctacgtgg aacttgagaa gatcatcctg
     3541 agcgagcgca agaatgtgcc tatcgagttt ccagtgatcg atcgcaaacg tctcctccag
     3601 ctcgtgcgtg agaatcagct gcagctcgac gagaacgagc tgcctcatgc tgtgcacttc
     3661 cttaacgagt caggtgtcct cctgcacttc caggacccgg ctctccagct gagtgacctc
     3721 tatttcgtgg agccaaagtg gctctgtaaa attatggctc aaatcctcac tgtcaaggta
     3781 gagggctgtc caaagcaccc aaagggcatc atctcgcgtc gcgacgtgga aaagtttctc
     3841 tctaagaagc gcaagtttcc caagaactac atgagccagt actttaaact gttggagaag
     3901 ttccagatcg ctctgcctat cggcgaagag tacctgttgg ttccttcctc cctgtccgat
     3961 caccgccccg tgattgagct gcctcactgc gagaacagcg agattatcat tcgcctatac
     4021 gagatgccgt actttccgat gggcttctgg tcaaggctca tcaatcgtct gcttgaaatc
     4081 tccccatata tgctgagcgg tcgtgaacgc gctctgaggc caaaccggat gtactggcgc
     4141 caggggattt acttgaactg gtcccctgag gcttattgtc tggtgggctc ggaagtcctg
     4201 gataaccacc ccgaatcatt cctgaagatc accgttcctt cctgccgtaa gggttgcatt
     4261 ctattgggac aggtggtgga tcatatcgat tccctgatgg aggagtggtt ccctggcctg
     4321 ttggagatcg acatctgtgg cgagggtgag accctgctga agaagtgggc actctacagt
     4381 ttcaatgacg gcgaagaaca ccagaagatt ctgctcgacg acctcatgaa gaaggccgag
     4441 gagggcgacc tgctcgtgaa ccccgaccag cctaggctga ccatccccat ctcccagatt
     4501 gcacccgacc ttatcctcgc tgacctgcct cgcaacatca tgctgaacaa tgacgagctg
     4561 gagtttgaac aagcacccga gttcctgcta ggcgacggct ctttcggttc agtttaccgt
     4621 gctgcttacg agggtgaaga ggttgccgtg aagattttta acaagcacac ttcgctccgt
     4681 ctgctgcgtc aggagcttgt ggttctctgc cacctgcacc acccttccct gatctccctg
     4741 ctggccgccg gcattaggcc tcgcatgcta gtgatggaac tggccagtaa gggttcattg
     4801 gaccgcctgc tgcagcagga caaggctagc ctgacccgta cgctccagca ccgtatcgcc
     4861 ctgcatgtgg ccgacggact gcgctacttg cacagtgcca tgatcattta tcgcgatctg
     4921 aagcctcata atgttcttct gttcacgctc tacccaaacg cagctatcat tgccaaaatt
     4981 gccgattacg gcatagccca gtactgctgt cgtatgggta tcaagactag cgagggtaca
     5041 cctgggttcc gcgctcccga agtcgcacgc gggaacgtga tctacaacca gcaggccgac
     5101 gtgtactcgt ttggcctgct cctgtatgac attctcacta ccggaggccg gattgtggag
     5161 ggcctgaaat ttcccaacga gttcgacgag ctggagatcc agggcaagct gcctgacccc
     5221 gtgaaggagt acggttgtgc accctggcct atggtggaga agctcattaa gcagtgtctg
     5281 aaggagaacc ctcaggaacg ccccacaagc gcccaggtgt tcgacattct aaactctgca
     5341 gagttagtgt gtctgactag gcgtatctta ctcccgaaga acgtgatcgt tgagtgcatg
     5401 gtggcaacgc accacaactc gcgtaacgct agcatctggt taggatgtgg gcataccgat
     5461 cgtggccagc tgagtttcct cgacctcaac accgagtaaa gcttgcggcc gcactcgagc
     5521 accaccacca ccaccactaa cctaggtagc tgagcgcatg caagctgatc cgggttatta
     5581 gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta
     5641 ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg
     5701 attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag
     5761 gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg
     5821 attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat
     5881 attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt
     5941 tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag
     6001 agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
     6061 ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
     6121 attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat
     6181 ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga
     6241 caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa
     6301 tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg
     6361 cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt
     6421 ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg
     6481 tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg
     6541 tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa
     6601 ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg
     6661 tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt
     6721 gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc
     6781 ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag
     6841 cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat
     6901 tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata acaagccttt
     6961 tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgagg tttaaacgct
     7021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7621 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8941 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9001 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9061 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9121 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9181 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9241 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9301 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9361 tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg
     9421 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
//
