LOCUS       sequence-52215-e        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/Dvl1_1_670
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5498..5515
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5552..6909
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5568..6909)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7001..7018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7172..7760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7252..7271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7931..8722,8723..8791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8554..8573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8792..8896)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8923..9378
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9010..9029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9220..9241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccatggctga aaccaagatt
     3481 atctaccaca tggacgagga agagactcca tacctcgtaa agctgccggt tgctccagaa
     3541 cgtgtcactc tggctgactt caagaacgtc ctgagcaacc gcccggttca cgcatacaaa
     3601 ttcttcttta agtcaatgga ccaggacttt ggcgtagtta aggaggaaat cttcgacgat
     3661 aacgcaaagc tgccatgctt taacggccgt gtagtatctt ggctggtttt agctgaaggt
     3721 gcacacagcg acgctggctc ccaaggcacc gatagccaca ctgatctgcc gccacctctc
     3781 gaacgcaccg gcggtatagg cgactctcgt ccgccgagct tccacccaaa cgtagcttct
     3841 tcgcgtgacg gtatggataa tgaaaccggc accgaatcta tggtctctca ccgccgcgaa
     3901 cgtgcccgtc gccgtaaccg tgaagaggcc gctcgcacca acggtcaccc acgtggtgac
     3961 cgtcgccgtg atgtaggcct gccgcctgac agcgcttcca ccgctctgtc ctctgaactg
     4021 gaaagctcta gcttcgtgga ttctgacgaa gacggtagca ccagccgcct gagcagctcc
     4081 accgaacaat ccacttcttc ccgtctgatc cgcaaacata aacgtcgccg tcgcaagcag
     4141 cgcctccgcc aggctgaccg tgcgtcctct ttctcctcca tcaccgactc caccatgagc
     4201 ctgaacatcg ttactgtgac cttgaacatg gagcgtcacc atttcctggg cattagcatc
     4261 gttggccaaa gcaacgatcg cggcgacggt ggtatctata taggctccat catgaagggc
     4321 ggcgccgttg ccgcggacgg tcgcattgaa ccgggtgata tgctcctgca ggtgaacgat
     4381 gtcaatttcg agaacatgag caacgacgat gcagttcgtg ttctgcgtga aatcgttagc
     4441 cagactggcc cgatctctct gaccgtagct aagtgttggg acccgactcc tcgttcctat
     4501 ttcaccgttc cacgtgcaga cccggtacgc ccgattgacc cggcagcctg gctgagccac
     4561 actgctgctc tgaccggtgc actgccacgt tacgaactcg aagaagcacc gctgactgtg
     4621 aagtccgata tgagcgctgt agttcgtgta atgcaactgc cagattccgg tctcgaaatc
     4681 cgtgaccgta tgtggctgaa gatcactatc gctaacgccg ttatcggcgc tgacgtagtt
     4741 gactggctct acacccatgt agaaggcttt aaggagcgtc gtgaagcacg taaatacgca
     4801 tcctcgctgc ttaaacacgg ctttctgcgc cacaccgtaa acaagataac cttctctgaa
     4861 cagtgctact atgtgttcgg cgacctctgt agcaatctgg ccactctgaa cctgaactcc
     4921 ggcagctccg gtacttctga ccaggatacc ttagctccgc ttcctcaccc tgccgcaccg
     4981 tggccattag gccagggcta tccatatcag tacccgggtc ctccgccttg tttcccgccg
     5041 gcttaccagg acccaggttt tagctacggc tctggttcta ccggtagcca gcagtccgag
     5101 ggcagcaaat cctctggctc cactcgctct agccgccgtg ctccaggccg tgagaaagaa
     5161 cgccgtgctg ccggtgctgg cggtagcggc tctgaatccg atcacactgc tccttctggt
     5221 gtaggtagct cctggcgtga gcgccctgct ggtcagctga gccgtggttc cagcccacgt
     5281 tcccaagcta gcgctactgc tccgggcttg ccgccaccgc acccgactac taaggcatat
     5341 accgtagttg gtggtccgcc aggtggtcct ccagttcgtg agctggccgc cgttcctcct
     5401 gaactgaccg gttcccgtca gagctttcag aaagctatgg gtaacccgtg tgaatttttt
     5461 gttgacatca tgtgataagc ttgcggccgc actcgagcac caccaccacc accactaacc
     5521 taggtagctg agcgcatgca agctgatccg ggttattagt acatttatta agcgctagat
     5581 tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta
     5641 taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg
     5701 aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag
     5761 ggttgttttt ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa
     5821 cttgccaaat cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt
     5881 aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg
     5941 ttagtgtaca attgactcga cgtaaacacg ttaaatagag cttggacata tttaacatcg
     6001 ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt
     6061 gcttccgaag acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg
     6121 tccgcgatca aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg
     6181 gcgggtttca atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt
     6241 gcaggcggtg gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat
     6301 gataaatcta ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt
     6361 ggtggcggtg atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc
     6421 acctcaacta ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg
     6481 cgattttttt cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg
     6541 ggttgaggtt ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt
     6601 ggtggtggag gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt
     6661 ataatttgtt ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct
     6721 ggctgcacaa cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata
     6781 ttataattgg aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt
     6841 accgtgccga tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc
     6901 aagctcggat cgatcccgca cgccgataac aagccttttc atttttacta cagcattgta
     6961 gtggcgagac acttcgctgt cgtcgaggtt taaacgcttc ctcgctcact gactcgctgc
     7021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7861 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7921 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7981 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8041 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8101 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8161 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8221 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8281 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8341 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8401 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8461 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8521 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8581 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8641 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8701 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8761 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8821 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8881 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     8941 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9001 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9061 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9121 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9181 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9241 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9301 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9361 tattaacgtt tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     9421 cggtgcgggc ctcttcgcta ttacgcca
//
